Iba1 Antikörper (AA 51-147)

Produktnummer ABIN685477

Dieses unabhängig validierte Kaninchen Polyklonal-Antikörper erkennt spezifisch Iba1 in WB, ELISA, IHC (p), FACS, IF (cc), IF (p) und IHC (fro). Er zeigt eine Reaktivität gegenüber Human, Ratte und Maus und wurde in 7+ Publikationen erwähnt.

Kurzübersicht für Iba1 Antikörper (AA 51-147) (ABIN685477)

Target: Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Iba1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-Iba1 Antikörper

Bindungsspezifität: AA 51-147

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Pig

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human AIF1

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Independent Validation (IF)

Validierung #029613 (Immunofluorescence)

by: Reveal Biosciences

No.: #029613

Datum: 21.02.2014

Chargennummer: 980993W

Validierte Anwendung: Immunofluorescence

Positivkontrolle: Mouse spleen

Negativkontrolle: Mouse duodenum

Bewertung: Strong signal was observed in the positive control tissue, while no signal was observed in the negative control tissue.

Validierungsbilder

Figure 1: AIF1 staining on mouse spleen (red), counterstained with DAPI (blue).

Figure 2: AIF1 staining on mouse duodenum (red), counterstained with DAPI (blue).

Figure 3: Isotype control staining on mouse spleen (red), counterstained with DAPI (blue).

Figure 4: Secondary only staining on mouse spleen (red), counterstained with DAPI (blue).

Protokoll

Primärantikörper:

• Antibody: AIF1
• Catalog number: ABIN685477
• Lot number: 980993W

Sekundärantikörper:

• Antibody: Alexa Fluor 546 Donkey Anti Rabbit
• Lot number: 1218269

Full Protocol:

• Frozen, OCT embedded tissues were cut 5 µm and mounted on positive charged slides.
• Sections were air dried for 30 min prior to fix in 10% neutral buffered formalin for 10 min.
• Fixed slides were rinsed three times in PBS for 5 min each at RT.
• Sections were blocked in 1 X PBS / 3% donkey serum / 0.1% Triton X-100 for 10 min at RT.
• Sections were incubated with primary antibody diluted 1:100 in Universal Antibody Dilution Buffer (Electron Microscopy Sciences, 25886-05) overnight at 4°C.
• Sections were rinsed three times in PBS for 5 min each at RT.
• Sections were incubated with secondary antibody diluted 1:200 in Universal Antibody Dilution Buffer (Electron Microscopy Sciences, 25886-05) for 120 min at RT in dark.
• Sections were mounted on slides with FluoroGel II with DAPI (Electron Microscopy Sciences).
• Stained sections were imaged with a 3D Histech Pannoramic SCAN 150.

Anmerkungen: None

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Referenzen für anti-Iba1 Antikörper (ABIN685477)

Ko, Shie, Lin, Chen, Yao, Chen: "Biodegradable Bisvinyl Sulfonemethyl-crosslinked Gelatin Conduit Promotes Regeneration after Peripheral Nerve Injury in Adult Rats." in: Scientific reports, Vol. 7, Issue 1, pp. 17489, (2018) (PubMed).

Hsu, Yao, Hsu, Lin, Chen, Chen: "Effects of Taxol on Regeneration in a Rat Sciatic Nerve Transection Model." in: Scientific reports, Vol. 7, pp. 42280, (2017) (PubMed).

Fu, Xia, Liu, Wang, Wang, Shi, Song, Song, Song et al.: "The acute exposure of tetrachloro-p-benzoquinone (a.k.a. chloranil) triggers inflammation and neurological dysfunction via Toll-like receptor 4 signaling: The protective role of melatonin ..." in: Toxicology, Vol. 381, pp. 39-50, (2017) (PubMed).

Hu, Kodithuwakku, Zhou, Li, Han, Fang, Liu, Li: "Levo-Corydalmine Alleviates Neuropathic Cancer Pain Induced by Tumor Compression via the CCL2/CCR2 Pathway." in: Molecules (Basel, Switzerland), Vol. 22, Issue 6, (2017) (PubMed).

Furukawa, Nagaike, Ozaki: "Databases for technical aspects of immunohistochemistry." in: Journal of toxicologic pathology, Vol. 30, Issue 1, pp. 79-107, (2017) (PubMed).

Kouam, Yuan, Njayou, He, Tsayem, Oladejo, Song, Moundipa, Gao: "Induction of Mkp-1 and Nuclear Translocation of Nrf2 by Limonoids from Khaya grandifoliola C.DC Protect L-02 Hepatocytes against Acetaminophen-Induced Hepatotoxicity." in: Frontiers in pharmacology, Vol. 8, pp. 653, (2017) (PubMed).

Ogawa, Onozato, Okuhara, Nagasawa, Tamura, Hayashi: "Spontaneous cutaneous soft tissue sarcoma with differentiation into fibroblasts in a Sprague-Dawley rat." in: Journal of toxicologic pathology, Vol. 29, Issue 2, pp. 119-24, (2016) (PubMed).

Details zu Iba1

Target: Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))

Andere Bezeichnung: AIF1

Hintergrund:

Synonyms: IBA1, IRT1, AIF-1, IRT-1, Allograft inflammatory factor 1, Ionized calcium-binding adapter molecule 1, Protein G1, AIF1, G1

Background: Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation.

Gen-ID: 199

UniProt: P55008

Pathways: Smooth Muscle Cell Migration