Clusterin Antikörper (AA 331-449)

Produktnummer ABIN685342

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Clusterin in ELISA, IHC (p), FACS, IHC (fro), IF (cc) und IF (p). Er zeigt eine Reaktivität gegenüber Human, Ratte und Maus und wurde in 2+ Publikationen erwähnt.

Kurzübersicht für Clusterin Antikörper (AA 331-449) (ABIN685342)

Target: Clusterin (CLU)

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Clusterin Antikörper ist unkonjugiert

Applikation: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Produktdetails anti-Clusterin Antikörper

Bindungsspezifität: AA 331-449

Kreuzreaktivität: Human, Maus, Ratte

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Apolipoprotein J

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Referenzen für anti-Clusterin Antikörper (ABIN685342)

Keck, Androsova, Gualtieri, Walker, von Rüden, Russmann, Deeg, Hauck, Krause, Potschka: "A systems level analysis of epileptogenesis-associated proteome alterations." in: Neurobiology of disease, Vol. 105, pp. 164-178, (2017) (PubMed).

Yu, Chen, Wang, Zhou, Luo, Xu, Shen, Yin, Tao, Xiao, Xiao, Lü, Wang: "Time-dependent decrease of clusterin as a potential cerebrospinal fluid biomarker for drug-resistant epilepsy." in: Journal of molecular neuroscience : MN, Vol. 54, Issue 1, pp. 1-9, (2014) (PubMed).

Details zu Clusterin

Target: Clusterin (CLU)

Andere Bezeichnung: Apolipoprotein J

Hintergrund:

Synonyms: CLI, AAG4, APOJ, CLU1, CLU2, KUB1, SGP2, APO-J, SGP-2, SP-4, TRPM2, TRPM-2, NA1/NA2, Clusterin, Aging-associated gene 4 protein, Apolipoprotein J, Complement cytolysis inhibitor, Complement-associated protein SP-4,4, Ku7-binding protein 1, Testosterone-repressed prostate message 2, CLU

Background: Isoform 1 functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress-induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC7. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. Secreted isoform 1 protects cells against apoptosis and against cytolysis by complement. Intracellular isoforms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Nuclear isoforms promote apoptosis. Mitochondrial isoforms suppress BAX-dependent release of cytochrome c into the cytoplasm and inhibit apoptosis. Plays a role in the regulation of cell proliferation.

Gen-ID: 1191

UniProt: P10909

Pathways: Apoptose, Negative Regulation of intrinsic apoptotic Signaling