c-FOS Antikörper (pThr325)

Produktnummer ABIN684928

Produktdetails anti-c-FOS Antikörper

Target: c-FOS (c-Fos) (c-Fos)

Bindungsspezifität: pThr325

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser c-FOS Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)

Spezifität: This phosphorylation site is homologous to Thr325 in Mouse and Rat.

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Dog,Sheep,Pig,Chicken

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human c-Fos around the phosphorylation site of Thr325

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Referenzen für anti-c-FOS Antikörper (ABIN684928)

Tavares, Pathak: "Helicobacter pylori Secreted Protein HP1286 Triggers Apoptosis in Macrophages via TNF-Independent and ERK MAPK-Dependent Pathways." in: Frontiers in cellular and infection microbiology, Vol. 7, pp. 58, (2017) (PubMed).

Details zu c-FOS

Target: c-FOS (c-Fos) (c-Fos)

Andere Bezeichnung: c-Fos (c-Fos Produkte)

Synonyme: AP-1 antikoerper, C-FOS antikoerper, p55 antikoerper, c-fos antikoerper, D12Rfj1 antikoerper, cFos antikoerper, cFOS antikoerper, fos antikoerper, Fos proto-oncogene, AP-1 transcription factor subunit antikoerper, FBJ osteosarcoma oncogene antikoerper, proto-oncogene c-Fos-like antikoerper, FOS antikoerper, Fos antikoerper, LOC101078177 antikoerper

Hintergrund:

Synonyms: p55, AP-1, C-FOS, Proto-oncogene c-Fos, Cellular oncogene fos, G0/G1 switch regulatory protein 7, FOS, G0S7

Background: Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation. In growing cells, activates phospholipid synthesis, possibly by activating CDS1 and PI4K2A. This activity requires Tyr-dephosphorylation and association with the endoplasmic reticulum.

Gen-ID: 2353

UniProt: P01100

Pathways: S100 Proteine