SMAD2 Antikörper (pSer465)

Produktnummer ABIN678818

Der Kaninchen Polyklonal Anti-SMAD2-Antikörper wurde für WB, ELISA, FACS, IHC (p), IF (cc), IF (p) und IHC (fro) validiert. Er ist geeignet, SMAD2 in Proben von Human zu detektieren.

Kurzübersicht für SMAD2 Antikörper (pSer465) (ABIN678818)

Target: SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SMAD2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-SMAD2 Antikörper

Bindungsspezifität: pSer465

Kreuzreaktivität: Human

Homologie: Mouse,Rat,Dog,Cow,Horse,Chicken

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human Smad2 around Ser465

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Details zu SMAD2

Target: SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))

Andere Bezeichnung: Smad2

Hintergrund:

Synonyms: Smad2Ser465, p-Smad2Ser465, Smad2S465, Mothers against decapentaplegic homolog 2, SMAD 2, Mothers against DPP homolog 2, Smad2, hMAD 2, hSMAD2, JV18 1, JV18, JV181, MAD, MAD Related Protein 2, MADH2, MADR2, MGC22139, MGC34440, Mothers Against Decapentaplegic Homolog 2, mothers against DPP homolog 2, SMAD 2, SMAD, SMAD2, SMAD2_HUMAN.

Background: SMAD2 or Mothers against decapentaplegic homolog 2 is a polypeptide that, as its name describes, is a homolog of the Drosophila gene: "Mothers against decepentaplegic". It belongs to the SMAD family of proteins, which belong to the TGF-Beta superfamily of modulators. Like many other TGF-Beta family members SMAD2 is involved in cell signalling. SMAD2 modulates signals of activin and TGF-Beta's. It interacts with SMAD anchor for receptor activation (SARA). The binding of ligands causes the phosphorylation of the SMAD2 protein and the dissociation from SARA and the association with SMAD4. It is subsequently transferred to the nucleus where it forms complexes with other proteins and acts as a transcription factor. SMAD2 is a receptor regulated SMAD (R-SMAD) and is activated by bone morphogenetic protein type 1 receptor kinase. Smad2 (Mothers against decapentaplegic homolog 2, SMAD 2, Mothers against DPP homolog 2,)

Gen-ID: 4087

Pathways: Zellzyklus, Hormone Transport, Chromatin Binding, Protein targeting to Nucleus