S100A8 Antikörper

Produktnummer ABIN6757634

Der Ziege Polyklonal Anti-S100A8-Antikörper wurde für WB, ELISA und ICC validiert. Er ist geeignet, S100A8 in Proben von Maus zu detektieren.

Kurzübersicht für S100A8 Antikörper (ABIN6757634)

Target: S100A8 (S100 Calcium Binding Protein A8 (S100A8))

Reaktivität: Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser S100A8 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

Produktdetails anti-S100A8 Antikörper

Spezifität: Recognizes murine calgranulin A (S100 calcium-binding Protein A8) at ~10kD. In direct ELISAs and western blots, shows less than 5 % cross-reactivity with Recombinant, Murine S100A9, Recombinant, Murine S100A10 and Recombinant, Human S100B.

Aufreinigung: Immunoaffinity purified

Immunogen: E. coli-derived, Recombinant, Murine S100A8 Calgranulin A.

Type of Immunogen: Recombinant protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (0.5 - 1 μg/mL), ICC, WB (0.1 - 0.2 μg/mL)

Usage: Suitable for use in Western Blot, Immunocytochemistry and ELISA. Between each step, cells were washed with PBS containing 0.1 % BSA. Western Blot: 0.1-0.2 μg/mL. The detection limit is approximately 25 ng/lane and 5 ng/lane under non-reducing and reducing conditions, respectively. Direct ELISA: 0.5-1 μg/mL with the appropriate secondary reagents to detect mouse S100A8. The detection limit for Recombinant, Murine S100A8 is approximately 0.2 ng/well.

Kommentare:

Target Species of Antibody: Mouse

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, 5 % trehalose, 40 % glycerol.

Handhabung: avoid freeze thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Details zu S100A8

Target: S100A8 (S100 Calcium Binding Protein A8 (S100A8))

Andere Bezeichnung: S100A8 / MRP8

Hintergrund: Name/Gene ID: S100A8

Synonyms: S100A8, 60B8AG, CFAG, CAGA, Calgranulin A, Calgranulin-A, Calprotectin L1L subunit, Cystic fibrosis antigen, CGLA, L1Ag, NIF, p8, Protein S100-A8, MRP8, Urinary stone protein band A, CP-10, MA387, MRP-8

Gen-ID: 6279

UniProt: P05109

Pathways: Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, S100 Proteine