FZD4 Antikörper (Extracellular Domain)

Produktnummer ABIN6757418

Dieses Anti-FZD4-Antikörper ist ein Ratte Monoklonal-Antikörper zur Detektion von FZD4 in WB, FACS und ICC. Geeignet für Human und Maus.

Kurzübersicht für FZD4 Antikörper (Extracellular Domain) (ABIN6757418)

Target: FZD4 (Frizzled Family Receptor 4 (FZD4))

Reaktivität: Human, Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser FZD4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Produktdetails anti-FZD4 Antikörper

Bindungsspezifität: Extracellular Domain

Spezifität: Recognizes mouse Frizzled-4. Shows no cross-reactivity with rmFrizzled-1, -2, 3, -6, -7 -8 or rhFrizzled-5. Species cross-reactivity: Human. Detects Frizzled-4 by Flow Cytometry and Immunohistochemistry on mouse differentiated embryoid bodies derived from the mouse embryonic cell line, D3. Also recognizes human Frizzled-4 by Flow Cytometry and Immunohistochemistry on HEK 293 cells.

Aufreinigung: Protein G purified

Immunogen: NS0-derived rmFrizzled-4 extracellular domain.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Approved: Flo (100 μg/mL), ICC (10 μg/mL), WB (1 - 2 μg/mL)

Usage: Suitable for use in Flow Cytometry, Immunocytochemistry and Western Blot. Flow Cytometry: 100 μg/mL. Add 10 μL to 1-2.5x10^5 cells. Total reaction volume should not exceeding 200 μL. Immunocytochemistry: 10 μg/mL in 4 % paraformaldehyde-fixed cells. Western Blot:1-2 μg/mL. The detection limit is ~5 ng/lane under non-reducing and reducing conditions.

Kommentare:

Target Species of Antibody: Mouse

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: Sterile PBS

Konzentration: Lot specific

Buffer: PBS,  pH 7.2, 5 % trehalose.

Handhabung: avoid freeze thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Details zu FZD4

Target: FZD4 (Frizzled Family Receptor 4 (FZD4))

Andere Bezeichnung: FZD4 / Frizzled 4

Hintergrund: Name/Gene ID: FZD4
Subfamily: Frizzled
Family: GPCR

Synonyms: FZD4, CD344, CD344 antigen, EVR1, Frizzled family receptor 4, Frizzled-4, Fz-4, Fz4, HFz4, Exudative vitreoretinopathy 1, FEVR, FZD4S, FzE4, Frizzled 4, Frizzled homolog 4, WNT receptor frizzled-4

Gen-ID: 8322

UniProt: Q9ULV1

Pathways: WNT Signalweg, Hormone Transport, Sensory Perception of Sound