LRP5 Antikörper (C-Term)

Produktnummer ABIN6757408

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch LRP5 in WB und IF. Er zeigt eine Reaktivität gegenüber Human und Maus.

Kurzübersicht für LRP5 Antikörper (C-Term) (ABIN6757408)

Target: LRP5 (Low Density Lipoprotein Receptor-Related Protein 5 (LRP5))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser LRP5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Produktdetails anti-LRP5 Antikörper

Bindungsspezifität: C-Term

Spezifität: Recognizes human LRP5 protein. Western Blot tests using negative control mock pSEC-tag-transfected 293T cell lysates did not yield the target band. Species cross-reactivity: Mouse.

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide derived from the C-terminal region of the human LRP5 protein. This intracellular region is identical to the corresponding mouse and rat LRP5 amino acid sequences.

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IF (2 - 3 μg/mL), WB (1 - 3 μg/mL)

Usage: Suitable for use in Immunofluorescence and Western Blot. Immunofluorescence: 2-3 μg/mL, Note: For optimal results in immunofluorescence assays, cells should be fixed in 4 % paraformaldehyde/0.5 % Triton X-100/PBS for 10 minutes, permeabilized with 0.5 % Triton X-100/PBS for 5 minutes, and blocked with 5 % BSA/PBS for 30 minutes. Western Blot: 1-3 μg/mL, Single band at ~200kD. Positive control: Mouse LRP5-pSEC-tag-transfected 293T cells, MCF-7 human breast adenocarcinoma, T47D human breast ductal carcinoma, and NIH-3T3 mouse embryo fibroblast lysates.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide, 40 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu LRP5

Target: LRP5 (Low Density Lipoprotein Receptor-Related Protein 5 (LRP5))

Andere Bezeichnung: LRP5

Hintergrund: Name/Gene ID: LRP5

Synonyms: LRP5, EVR1, Exudative vitreoretinopathy 1, LRP7, OPTA1, OPPG, OPS, LR3, LRP-5, VBCH2, BMND1, EVR4

Gen-ID: 4041

UniProt: O75197

Pathways: WNT Signalweg, Stem Cell Maintenance, Positive Regulation of fat Cell Differentiation