PAX7 Antikörper (AA 201-250)

Produktnummer ABIN6746020

Dieses Anti-PAX7-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von PAX7 in WB. Geeignet für Human, Maus, Ratte, Zebrafisch (Danio rerio), Huhn, Hund, Meerschweinchen, Rind (Kuh), Kaninchen, Fledermaus, Affe und Xenopus laevis. Dieses Primary Antibody wurde in 3+ Publikationen zitiert.

Kurzübersicht für PAX7 Antikörper (AA 201-250) (ABIN6746020)

Target: PAX7 (Paired Box 7 (PAX7))

Reaktivität: Human, Maus, Ratte, Zebrafisch (Danio rerio), Huhn, Hund, Meerschweinchen, Rind (Kuh), Kaninchen, Fledermaus, Affe, Xenopus laevis

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PAX7 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-PAX7 Antikörper

Bindungsspezifität: AA 201-250

Spezifität: Human PAX7

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Guinea pig, Chicken, Xenopus, Zebrafish (100%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa201-250 of human Pax7 (NP_001178913). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Guinea pig, Turkey, Zebra finch, Chicken, Xenopus, Salmon, Zebrafish (100%), Goat, Horse, Platypus, Lizard, Stickleback (85%), Drosophila, Beetle (84%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Not recommended for: IF

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled water

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Referenzen für anti-PAX7 Antikörper (ABIN6746020)

Ku, Meier: "Phosphorylation of paxillin via the ERK mitogen-activated protein kinase cascade in EL4 thymoma cells." in: The Journal of biological chemistry, Vol. 275, Issue 15, pp. 11333-40, (2000) (PubMed).

Takayama, Tanaka, Nagai, Okada et al.: "Adenovirus-mediated overexpression of C-terminal Src kinase (Csk) in type I astrocytes interferes with cell spreading and attachment to fibronectin. Correlation with tyrosine phosphorylations of ..." in: The Journal of biological chemistry, Vol. 274, Issue 4, pp. 2291-7, (1999) (PubMed).

Salgia, Li, Lo, Brunkhorst, Kansas, Sobhany, Sun, Pisick, Hallek, Ernst: "Molecular cloning of human paxillin, a focal adhesion protein phosphorylated by P210BCR/ABL." in: The Journal of biological chemistry, Vol. 270, Issue 10, pp. 5039-47, (1995) (PubMed).

Details zu PAX7

Target: PAX7 (Paired Box 7 (PAX7))

Andere Bezeichnung: PAX7

Hintergrund: Name/Gene ID: PAX7

Synonyms: PAX7, HUP1, Paired box 7, Paired box gene 7, Paired box homeotic gene 7, Paired box protein Pax-7, RMS2, Paired domain gene 7, PAX7 transcriptional factor, PAX7B

Gen-ID: 5081

NCBI Accession: NP_001178913

UniProt: P23759