DDAH2 Antikörper (N-Term)

Produktnummer ABIN6744442

Dieses Anti-DDAH2-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von DDAH2 in WB, IHC und IHC (p). Geeignet für Human, Maus, Ratte, Hund, Pferd, Kaninchen und Fledermaus.

Kurzübersicht für DDAH2 Antikörper (N-Term) (ABIN6744442)

Target: DDAH2 (Dimethylarginine Dimethylaminohydrolase 2 (DDAH2))

Reaktivität: Human, Maus, Ratte, Hund, Pferd, Kaninchen, Fledermaus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser DDAH2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-DDAH2 Antikörper

Bindungsspezifität: N-Term

Marke: IHC-plus™

Spezifität: Human DDAH2

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Horse (100%) Bovine (92%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human DDAH2 (O95865, NP_039268). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Galago, Marmoset, Mouse, Rat, Panda, Dog, Bat, Horse (100%), Monkey, Bovine (92%), Elephant (85%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled water

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu DDAH2

Target: DDAH2 (Dimethylarginine Dimethylaminohydrolase 2 (DDAH2))

Andere Bezeichnung: DDAH2 / DDAH

Hintergrund: Name/Gene ID: DDAH2

Synonyms: DDAH2, DDAH, DDAH-2, DDAHII, G6a, Alternative name: NG30, G6a, Unknown, Dimethylargininase-2, NG30, Protein G6a, S-phase protein

Gen-ID: 23564

NCBI Accession: NP_039268

UniProt: O95865