GPSM2 Antikörper (AA 72-121)

Produktnummer ABIN6744437

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch GPSM2 in WB, IHC und IHC (p). Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte, Rind (Kuh), Hund, Pferd, Kaninchen, Zebrafisch (Danio rerio), Affe und Fledermaus.

Kurzübersicht für GPSM2 Antikörper (AA 72-121) (ABIN6744437)

Target: GPSM2 (G-Protein Signaling Modulator 2 (GPSM2))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Hund, Pferd, Kaninchen, Zebrafisch (Danio rerio), Affe, Fledermaus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GPSM2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-GPSM2 Antikörper

Bindungsspezifität: AA 72-121

Marke: IHC-plus™

Spezifität: Human GPSM2

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Zebrafish (100%) Xenopus (92%) Chicken (85%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa72-121 of human GPSM2 (P81274, NP_037428). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Opossum, Zebrafish (100%), Zebra finch, Platypus, Xenopus (92%), Hamster, Panda, Guinea pig, Turkey, Chicken (85%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Not recommended for: IF

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled water

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu GPSM2

Target: GPSM2 (G-Protein Signaling Modulator 2 (GPSM2))

Andere Bezeichnung: GPSM2 / LGN

Hintergrund: Name/Gene ID: GPSM2

Synonyms: GPSM2, DFNB82, LGN, PINS, CMCS, Mosaic protein LGN

Gen-ID: 29899

NCBI Accession: NP_037428

UniProt: P81274

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling