IGFBP4 Antikörper (AA 180-229)

Produktnummer ABIN6744434

Produktdetails anti-IGFBP4 Antikörper

Target: IGFBP4 (Insulin-Like Growth Factor Binding Protein 4 (IGFBP4))

Bindungsspezifität: AA 180-229

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Kaninchen, Schaf, Hund, Pferd, Ziege, Fledermaus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser IGFBP4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human IGFBP4

Homologie: Percent identity by BLAST analysis: Human, Mouse, Sheep, Dog, Bovine, Horse (100%) Pig, Guinea pig, Chicken (92%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa180-229 of human IGFBP4 (P22692, NP_001543). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Mouse, Rat, Sheep, Goat, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse (100%), Marmoset, Hamster, Pig, Guinea pig, Turkey, Zebra finch, Chicken, Lizard (92%), Opossum (85%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled water

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu IGFBP4

Target: IGFBP4 (Insulin-Like Growth Factor Binding Protein 4 (IGFBP4))

Andere Bezeichnung: IGFBP4 / IGFBP-4 (IGFBP4 Produkte)

Synonyme: IGFBP4 antikoerper, igfbp4 antikoerper, BP-4 antikoerper, HT29-IGFBP antikoerper, IBP4 antikoerper, IGFBP-4 antikoerper, AI875747 antikoerper, Deb2 antikoerper, IGF-BP4 antikoerper, IBP-4 antikoerper, insulin like growth factor binding protein 4 antikoerper, insulin-like growth factor-binding protein 4 antikoerper, insulin-like growth factor binding protein 4 antikoerper, IGFBP4 antikoerper, LOC100136305 antikoerper, LOC100194498 antikoerper, igfbp4 antikoerper, Igfbp4 antikoerper

Hintergrund: Name/Gene ID: IGFBP4

Synonyms: IGFBP4, BP-4, HT29-IGFBP, IGF-binding protein 4, IBP-4, IGFBP-4, IBP4

Gen-ID: 3487

NCBI Accession: NP_001543

UniProt: P22692

Pathways: WNT Signalweg, Myometrial Relaxation and Contraction, Regulation of Carbohydrate Metabolic Process