CBLN4 Antikörper (AA 125-174)

Produktnummer ABIN6741612

Produktdetails anti-CBLN4 Antikörper

Target: CBLN4 (Cerebellin 4 Precursor (CBLN4))

Bindungsspezifität: AA 125-174

Reaktivität: Human, Maus, Ratte, Affe, Rind (Kuh), Schwein, Kaninchen, Zebrafisch (Danio rerio), Hund, Meerschweinchen, Pferd, Xenopus laevis, Fledermaus, Huhn

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CBLN4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human CBLN4

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Pig, Guinea pig, Chicken, Xenopus, Zebrafish (100%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa125-174 of human CBLN4 (Q9NTU7, NP_542184). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Xenopus, Stickleback, Zebrafish (100%), Salmon, Smelt (92%), Lizard, Seabass (85%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled Water.

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu CBLN4

Target: CBLN4 (Cerebellin 4 Precursor (CBLN4))

Andere Bezeichnung: CBLN4 / Cerebellin 4 (CBLN4 Produkte)

Synonyme: zgc:172190 antikoerper, cerebellin-4 antikoerper, CBLNL1 antikoerper, AI848962 antikoerper, cerebellin 4 precursor antikoerper, cerebellin 4 precursor L homeolog antikoerper, cerebellin 4 precursor protein antikoerper, CBLN4 antikoerper, cbln4 antikoerper, cbln4.L antikoerper, Cbln4 antikoerper

Hintergrund: Name/Gene ID: CBLN4

Synonyms: CBLN4, CBLNL1, Cerebellin precursor-like 1, Cerebellin-4, Cerebellin 4 precursor, Cerebellin-like glycoprotein 1, DJ885A10.1

Gen-ID: 140689

NCBI Accession: NP_542184

UniProt: Q9NTU7