SLC25A28 Antikörper (AA 290-339)

Produktnummer ABIN6741603

Der Kaninchen Polyklonal Anti-SLC25A28-Antikörper wurde für WB, IHC und IHC (p) validiert. Er ist geeignet, SLC25A28 in Proben von Human, Ratte, Rind (Kuh) und Affe zu detektieren.

Kurzübersicht für SLC25A28 Antikörper (AA 290-339) (ABIN6741603)

Target: SLC25A28 (Solute Carrier Family 25, Member 28 (SLC25A28))

Reaktivität: Human, Ratte, Rind (Kuh), Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SLC25A28 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-SLC25A28 Antikörper

Bindungsspezifität: AA 290-339

Marke: IHC-plus™

Spezifität: Human SLC25A28

Homologie: Percent identity by BLAST analysis: Dog, Rabbit, Pig, Guinea pig (92%) Rat, Bovine (85%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa290-339 of human SLC25A28 (Q96A46, NP_112489). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset (100%), Galago, Mouse, Elephant, Panda, Dog, Rabbit, Horse, Pig, Guinea pig, Chicken (92%), Rat, Bovine, Bat, Opossum, Platypus (85%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled Water.

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu SLC25A28

Target: SLC25A28 (Solute Carrier Family 25, Member 28 (SLC25A28))

Andere Bezeichnung: SLC25A28

Hintergrund: Name/Gene ID: SLC25A28

Synonyms: SLC25A28, Mitoferrin 2, MRS4L, HMRS3/4, MFRN2, MRS3/4, Mitoferrin-2, NPD016

Gen-ID: 81894

NCBI Accession: NP_112489

UniProt: Q96A46

Pathways: Transition Metal Ion Homeostasis