LIN7C Antikörper (N-Term)

Produktnummer ABIN6741597

Der Kaninchen Polyklonal Anti-LIN7C-Antikörper wurde für WB, IHC und IHC (p) validiert. Er ist geeignet, LIN7C in Proben von Human, Ratte, Maus, Rind (Kuh), Hund, Meerschweinchen, Pferd, Kaninchen, Fledermaus, Huhn, Hamster, Affe und Schwein zu detektieren.

Kurzübersicht für LIN7C Antikörper (N-Term) (ABIN6741597)

Target: LIN7C (Lin-7 Homolog C (LIN7C))

Reaktivität: Human, Ratte, Maus, Rind (Kuh), Hund, Meerschweinchen, Pferd, Kaninchen, Fledermaus, Huhn, Hamster, Affe, Schwein

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser LIN7C Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-LIN7C Antikörper

Bindungsspezifität: N-Term

Marke: IHC-plus™

Spezifität: Human LIN7C

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Chicken (100%) Zebrafish (92%) Xenopus (85%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human LIN7C (Q9NUP9, NP_060832). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Lizard (100%), Salmon, Stickleback, Zebrafish (92%), Xenopus, Smelt (85%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled Water.

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu LIN7C

Target: LIN7C (Lin-7 Homolog C (LIN7C))

Andere Bezeichnung: LIN7C / VELI3

Hintergrund: Name/Gene ID: LIN7C

Synonyms: LIN7C, LIN-7 protein 3, LIN-7-C, VELI3, Protein lin-7 homolog C, Vertebrate lin-7 homolog 3, Lin-7 homolog C (C. elegans), LIN-7C, MALS-3, MALS3, Mammalian lin-seven protein 3, Veli-3

Gen-ID: 55327

NCBI Accession: NP_060832

UniProt: Q9NUP9

Pathways: Synaptic Membrane