NPY1R Antikörper (AA 180-229)

Produktnummer ABIN462232

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch NPY1R in WB, IHC und IHC (p). Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte, Pferd und Affe.

Kurzübersicht für NPY1R Antikörper (AA 180-229) (ABIN462232)

Target: NPY1R (Neuropeptide Y Receptor Y1 (NPY1R))

Reaktivität: Human, Maus, Ratte, Pferd, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NPY1R Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-NPY1R Antikörper

Bindungsspezifität: AA 180-229

Marke: IHC-plus™

Spezifität: Human NPY1R

Homologie: Percent identity by BLAST analysis: Human (100%) Sheep, Dog, Bovine, Rabbit, Pig, Guinea pig (92%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa180-229 of human NPY1R (P25929, NP_000900). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset (100%), Galago, Sheep, Elephant, Panda, Dog, Bovine, Rabbit, Pig, Guinea pig (92%), Hamster (85%), Mouse, Rat (78%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled Water.

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu NPY1R

Target: NPY1R (Neuropeptide Y Receptor Y1 (NPY1R))

Andere Bezeichnung: NPY1R

Hintergrund: Name/Gene ID: NPY1R
Subfamily: Neuropeptide
Family: GPCR

Synonyms: NPY1R, NPYY1, NPY-Y1 receptor, Neuropeptide Y receptor type 1, PY receptor Y1, Neuropeptide Y receptor Y1, Neuropeptide Y-Y1 receptor, Neuropeptide Y1 receptor, NPY1-R, NPYR

Gen-ID: 4886

NCBI Accession: NP_000900

UniProt: P25929

Pathways: Negative Regulation of Hormone Secretion, Feeding Behaviour