TRIM8 Antikörper (AA 36-85)

Produktnummer ABIN6741584

Dieses Anti-TRIM8-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von TRIM8 in WB, IHC und IHC (p). Geeignet für Human, Ratte, Maus, Hund, Rind (Kuh), Kaninchen, Schwein, Zebrafisch (Danio rerio), Hamster und Affe.

Kurzübersicht für TRIM8 Antikörper (AA 36-85) (ABIN6741584)

Target: TRIM8 (Tripartite Motif Containing 8 (TRIM8))

Reaktivität: Human, Ratte, Maus, Hund, Rind (Kuh), Kaninchen, Schwein, Zebrafisch (Danio rerio), Hamster, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TRIM8 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-TRIM8 Antikörper

Bindungsspezifität: AA 36-85

Marke: IHC-plus™

Spezifität: Human TRIM8 / GERP

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Pig (100%) Horse, Chicken, Xenopus (92%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa36-85 of human TRIM8 (Q9BZR9, NP_112174). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Panda, Dog, Bovine, Rabbit, Pig (100%), Horse, Turkey, Zebra finch, Chicken, Lizard, Xenopus (92%), Opossum (91%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 - 10 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled water

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu TRIM8

Target: TRIM8 (Tripartite Motif Containing 8 (TRIM8))

Andere Bezeichnung: TRIM8 / GERP

Hintergrund: Name/Gene ID: TRIM8
Family: Tripartite Motif

Synonyms: TRIM8, RING finger protein 27, Tripartite motif-containing 8, Tripartite motif containing 8, GERP, RNF27, Tripartite motif protein TRIM8

Gen-ID: 81603

NCBI Accession: NP_112174

UniProt: Q9BZR9