MAZ Antikörper (N-Term)

Produktnummer ABIN6741567

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch MAZ in WB, IHC und IHC (p). Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte und Hamster.

Kurzübersicht für MAZ Antikörper (N-Term) (ABIN6741567)

Target: MAZ (MYC-Associated Zinc Finger Protein (Purine-Binding Transcription Factor) (MAZ))

Reaktivität: Human, Maus, Ratte, Hamster

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MAZ Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-MAZ Antikörper

Bindungsspezifität: N-Term

Marke: IHC-plus™

Spezifität: Human MAZ

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat (100%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human MAZ (P56270, NP_002374). Percent identity by BLAST analysis: Human, Chimpanzee, Mouse, Rat, Hamster (100%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (10 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled water

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu MAZ

Target: MAZ (MYC-Associated Zinc Finger Protein (Purine-Binding Transcription Factor) (MAZ))

Andere Bezeichnung: MAZ

Hintergrund: Name/Gene ID: MAZ

Synonyms: MAZ, Pur-1, SAF-2, SAF-3, MAZI, ZF87, Zif87, ZNF801, Transcription factor Zif87, Zinc finger protein 801, PUR1, SAF-1

Gen-ID: 4150

NCBI Accession: NP_002374

UniProt: P56270

Pathways: Chromatin Binding