FUBP1 Antikörper (AA 540-589)
Kurzübersicht für FUBP1 Antikörper (AA 540-589) (ABIN6741561)
Target
Alle FUBP1 Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
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Bindungsspezifität
- AA 540-589
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Marke
- IHC-plus™
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Spezifität
- Human FUBP1
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Homologie
- Percent identity by BLAST analysis: Human, Rat, Bovine, Rabbit (100%) Dog (93%) Mouse, Guinea pig (92%) Chicken (85%).
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Aufreinigung
- Immunoaffinity purified
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Immunogen
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Synthetic peptide located between aa540-589 of human FUBP1 (Q96AE4, NP_003893). Percent identity by BLAST analysis: Human, Chimpanzee, Gibbon, Monkey, Marmoset, Rat, Elephant, Bovine, Rabbit, Pig (100%), Gorilla, Dog (93%), Mouse, Guinea pig, Stickleback (92%), Galago (87%), Turkey, Chicken, Platypus (85%), Bat (81%).
Type of Immunogen: Synthetic peptide -
Isotyp
- IgG
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Applikationshinweise
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Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:1562500. -
Kommentare
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Target Species of Antibody: Human
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Lyophilized
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Rekonstitution
- Distilled water
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Konzentration
- Lot specific
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Buffer
- Lyophilized from PBS with 2 % sucrose
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Handhabung
- Avoid repeat freeze-thaw cycles.
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Lagerung
- 4 °C,-20 °C
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Informationen zur Lagerung
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Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
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