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FUBP1 Antikörper (AA 540-589)

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch FUBP1 in WB, IHC und IHC (p). Er zeigt eine Reaktivität gegenüber Human, Ratte, Rind (Kuh), Kaninchen, Schwein und Affe.
Produktnummer ABIN6741561

Kurzübersicht für FUBP1 Antikörper (AA 540-589) (ABIN6741561)

Target

Alle FUBP1 Antikörper anzeigen
FUBP1 (Far Upstream Element (FUSE) Binding Protein 1 (FUBP1))

Reaktivität

  • 46
  • 24
  • 22
  • 5
  • 5
  • 4
  • 4
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
Human, Ratte, Rind (Kuh), Kaninchen, Schwein, Affe

Wirt

  • 40
  • 6
  • 1
Kaninchen

Klonalität

  • 29
  • 18
Polyklonal

Konjugat

  • 32
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser FUBP1 Antikörper ist unkonjugiert

Applikation

  • 37
  • 24
  • 16
  • 14
  • 9
  • 7
  • 4
  • 4
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • Bindungsspezifität

    • 8
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 540-589

    Marke

    IHC-plus™

    Spezifität

    Human FUBP1

    Homologie

    Percent identity by BLAST analysis: Human, Rat, Bovine, Rabbit (100%) Dog (93%) Mouse, Guinea pig (92%) Chicken (85%).

    Aufreinigung

    Immunoaffinity purified

    Immunogen

    Synthetic peptide located between aa540-589 of human FUBP1 (Q96AE4, NP_003893). Percent identity by BLAST analysis: Human, Chimpanzee, Gibbon, Monkey, Marmoset, Rat, Elephant, Bovine, Rabbit, Pig (100%), Gorilla, Dog (93%), Mouse, Guinea pig, Stickleback (92%), Galago (87%), Turkey, Chicken, Platypus (85%), Bat (81%).

    Type of Immunogen: Synthetic peptide

    Isotyp

    IgG
  • Applikationshinweise

    Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

    Kommentare

    Target Species of Antibody: Human

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    Distilled water

    Konzentration

    Lot specific

    Buffer

    Lyophilized from PBS with 2 % sucrose

    Handhabung

    Avoid repeat freeze-thaw cycles.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
    Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
  • Target

    FUBP1 (Far Upstream Element (FUSE) Binding Protein 1 (FUBP1))

    Andere Bezeichnung

    FUBP1

    Hintergrund

    Name/Gene ID: FUBP1

    Synonyms: FUBP1, DNA helicase V, FBP, HDH V, FUSE-binding protein 1, FUBP

    Gen-ID

    8880

    NCBI Accession

    NP_003893

    UniProt

    Q96AE4
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