NRF1 Antikörper (AA 360-409)

Produktnummer ABIN462197

Der Kaninchen Polyklonal Anti-NRF1-Antikörper wurde für WB, IHC und IHC (p) validiert. Er ist geeignet, NRF1 in Proben von Human, Ratte, Rind (Kuh), Kaninchen, Pferd, Meerschweinchen, Zebrafisch (Danio rerio), Hund, Affe, Huhn, Schwein und Xenopus laevis zu detektieren.

Kurzübersicht für NRF1 Antikörper (AA 360-409) (ABIN462197)

Target: NRF1 (Nuclear Respiratory Factor 1 (NRF1))

Reaktivität: Human, Ratte, Rind (Kuh), Kaninchen, Pferd, Meerschweinchen, Zebrafisch (Danio rerio), Hund, Affe, Huhn, Schwein, Xenopus laevis

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NRF1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-NRF1 Antikörper

Bindungsspezifität: AA 360-409

Marke: IHC-plus™

Spezifität: Human NRF1

Keine Kreuzreaktivität: Maus

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig, Chicken, Xenopus, Zebrafish (100%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa360-409 of human NRF1 (Q16656, NP_005002). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Xenopus, Stickleback, Zebrafish (100%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (10 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled water

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu NRF1

Target: NRF1 (Nuclear Respiratory Factor 1 (NRF1))

Andere Bezeichnung: NRF1 / NRF-1

Hintergrund: Name/Gene ID: NRF1
Family: Transcription factor

Synonyms: NRF1, EWG, NRF-1, Nuclear respiratory factor 1, ALPHA-PAL

Gen-ID: 4899

NCBI Accession: NP_005002

UniProt: Q16656

Pathways: Regulation of Lipid Metabolism by PPARalpha