LIN28A Antikörper (N-Term)

Produktnummer ABIN6741558

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch LIN28A in WB, IHC und IHC (p). Er zeigt eine Reaktivität gegenüber Human, Rind (Kuh), Hund, Schwein und Schaf.

Kurzübersicht für LIN28A Antikörper (N-Term) (ABIN6741558)

Target: LIN28A (Lin-28 Homolog A (C. Elegans) (LIN28A))

Reaktivität: Human, Rind (Kuh), Hund, Schwein, Schaf

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser LIN28A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-LIN28A Antikörper

Bindungsspezifität: N-Term

Marke: IHC-plus™

Spezifität: Human LIN28A / LIN28

Homologie: Percent identity by BLAST analysis: Dog, Bovine, Pig (100%) Rat, Sheep (85%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human LIN28A (Q9H9Z2, NP_078950). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Galago, Marmoset, Panda, Dog, Cat, Bovine, Pig (100%), Rabbit (92%), Monkey, Mouse, Rat, Elephant (90%), Sheep, Guinea pig (85%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled water

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu LIN28A

Target: LIN28A (Lin-28 Homolog A (C. Elegans) (LIN28A))

Andere Bezeichnung: LIN28A / LIN28

Hintergrund: Name/Gene ID: LIN28A

Synonyms: LIN28A, CSDD1, Lin-28 homolog (C. elegans), LIN-28, Lin-28A, LIN28, Protein lin-28 homolog A, Lin-28 homolog A (C. elegans), ZCCHC1, RNA-binding protein LIN-28

Gen-ID: 79727

NCBI Accession: NP_078950

UniProt: Q9H9Z2

Pathways: Stem Cell Maintenance