T-Box 5 Antikörper (AA 174-223)

Produktnummer ABIN6741551

Dieses Anti-T-Box 5-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von T-Box 5 in WB, IHC und IHC (p). Geeignet für Human, Maus, Ratte, Hund, Meerschweinchen, Kaninchen, Rind (Kuh), Pferd, Zebrafisch (Danio rerio), Ziege, Schaf, Fledermaus, Hamster, Affe, Schwein, Huhn und Xenopus laevis.

Kurzübersicht für T-Box 5 Antikörper (AA 174-223) (ABIN6741551)

Target: T-Box 5 (TBX5)

Reaktivität: Human, Maus, Ratte, Hund, Meerschweinchen, Kaninchen, Rind (Kuh), Pferd, Zebrafisch (Danio rerio), Ziege, Schaf, Fledermaus, Hamster, Affe, Schwein, Huhn, Xenopus laevis

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser T-Box 5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-T-Box 5 Antikörper

Bindungsspezifität: AA 174-223

Marke: IHC-plus™

Spezifität: Human TBX5

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Chicken, Xenopus, Zebrafish (100%).

Aufreinigung: Protein A purified

Immunogen: Synthetic peptide located between aa174-223 of human TBX5 (Q99593, NP_000183). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Sheep, Goat, Hamster, Dolphin, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Xenopus, Newt, Stickleback, Medaka, Zebrafish (100%), Sea squirt (83%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled water

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu T-Box 5

Target: T-Box 5 (TBX5)

Andere Bezeichnung: TBX5

Hintergrund: Name/Gene ID: TBX5

Synonyms: TBX5, Hos, T-box protein 5, T-box 5

Gen-ID: 6910

NCBI Accession: NP_000183

UniProt: Q99593