Sp3 Antikörper (AA 287-336)

Produktnummer ABIN6741550

Der Kaninchen Polyklonal Anti-Sp3-Antikörper wurde für WB, IHC und IHC (p) validiert. Er ist geeignet, Sp3 in Proben von Human, Maus, Ratte, Meerschweinchen, Pferd, Kaninchen, Rind (Kuh), Hund, Fledermaus, Affe und Schwein zu detektieren.

Kurzübersicht für Sp3 Antikörper (AA 287-336) (ABIN6741550)

Target: Sp3 (Sp3 Transcription Factor (Sp3))

Reaktivität: Human, Maus, Ratte, Meerschweinchen, Pferd, Kaninchen, Rind (Kuh), Hund, Fledermaus, Affe, Schwein

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Sp3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-Sp3 Antikörper

Bindungsspezifität: AA 287-336

Marke: IHC-plus™

Spezifität: Human SP3

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig (100%) Chicken (92%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa287-336 of human SP3 (Q02447, NP_003102). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig (100%), Opossum, Chicken (92%), Turkey, Zebra finch, Platypus (85%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled water

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu Sp3

Target: Sp3 (Sp3 Transcription Factor (Sp3))

Andere Bezeichnung: SP3

Hintergrund: Name/Gene ID: SP3

Synonyms: SP3, SPR2, Transcription factor Sp3, Sp3 transcription factor, SPR-2, Specificity protein 3

Gen-ID: 6670

NCBI Accession: NP_003102

UniProt: Q02447