NOTCH4 Antikörper (AA 371-420)

Produktnummer ABIN6741546

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch NOTCH4 in WB, IHC und IHC (p). Er zeigt eine Reaktivität gegenüber Human, Rind (Kuh), Pferd, Meerschweinchen und Schwein.

Kurzübersicht für NOTCH4 Antikörper (AA 371-420) (ABIN6741546)

Target: NOTCH4 (Notch 4 (NOTCH4))

Reaktivität: Human, Rind (Kuh), Pferd, Meerschweinchen, Schwein

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NOTCH4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-NOTCH4 Antikörper

Bindungsspezifität: AA 371-420

Marke: IHC-plus™

Spezifität: Human NOTCH4

Homologie: Percent identity by BLAST analysis: Human, Bovine, Horse, Pig (100%) Guinea pig (85%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa371-420 of human NOTCH4 (Q99466-2). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Bovine, Horse, Pig (100%), Monkey, Marmoset (92%), Gibbon, Guinea pig (85%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled water

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu NOTCH4

Target: NOTCH4 (Notch 4 (NOTCH4))

Andere Bezeichnung: NOTCH4

Hintergrund: Name/Gene ID: NOTCH4

Synonyms: NOTCH4, HNotch4, Notch (Drosophila) homolog 4, Notch homolog 4 (Drosophila), Notch homolog 4, INT3, Notch 4

Gen-ID: 4855

UniProt: Q99466

Pathways: Notch Signalweg