MBD1 Antikörper (C-Term)

Produktnummer ABIN6736735

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch MBD1 in WB, IHC und IHC (p). Er zeigt eine Reaktivität gegenüber Human, Ratte, Hund, Rind (Kuh), Meerschweinchen, Pferd, Maus, Kaninchen, Affe, Fledermaus und Schwein.

Kurzübersicht für MBD1 Antikörper (C-Term) (ABIN6736735)

Target: MBD1 (Methyl-CpG Binding Domain Protein 1 (MBD1))

Reaktivität: Human, Ratte, Hund, Rind (Kuh), Meerschweinchen, Pferd, Maus, Kaninchen, Affe, Fledermaus, Schwein

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MBD1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-MBD1 Antikörper

Bindungsspezifität: C-Term

Marke: IHC-plus™

Spezifität: Human MBD1

Homologie: Percent identity by BLAST analysis: Human, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig (100%) Mouse, Rat (85%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human MBD1 (NP_056669). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Tamarin, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig (100%), Mouse, Rat (85%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (10 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu MBD1

Target: MBD1 (Methyl-CpG Binding Domain Protein 1 (MBD1))

Andere Bezeichnung: MBD1

Hintergrund: Name/Gene ID: MBD1

Synonyms: MBD1, CXXC3, RFT

Gen-ID: 4152

NCBI Accession: NP_056669

UniProt: Q9UIS9