E2F4 Antikörper (C-Term)

Produktnummer ABIN6736723

Der Kaninchen Polyklonal Anti-E2F4-Antikörper wurde für WB, IHC und IHC (p) validiert. Er ist geeignet, E2F4 in Proben von Human, Maus, Ratte, Rind (Kuh), Pferd, Kaninchen, Huhn, Hund, Schwein, Affe, Meerschweinchen, Zebrafisch (Danio rerio) und Fledermaus zu detektieren.

Kurzübersicht für E2F4 Antikörper (C-Term) (ABIN6736723)

Target: E2F4 (E2F Transcription Factor 4, P107/p130-Binding (E2F4))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Pferd, Kaninchen, Huhn, Hund, Schwein, Affe, Meerschweinchen, Zebrafisch (Danio rerio), Fledermaus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser E2F4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-E2F4 Antikörper

Bindungsspezifität: C-Term

Marke: IHC-plus™

Spezifität: Human E2F4

Homologie: Percent identity by BLAST analysis: Human, Mouse, Dog, Bovine, Rabbit, Pig, Chicken, Zebrafish (100%) Xenopus (92%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human E2F4 (Q16254, NP_001941). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Salmon, Zebrafish (100%), Xenopus, Stickleback (92%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled water

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu E2F4

Target: E2F4 (E2F Transcription Factor 4, P107/p130-Binding (E2F4))

Andere Bezeichnung: E2F4

Hintergrund: Name/Gene ID: E2F4

Synonyms: E2F4, E2F-4, p107/p130-binding protein, Transcription factor E2F4

Gen-ID: 1874

NCBI Accession: NP_001941

UniProt: Q16254

Pathways: Zellzyklus, Mitotic G1-G1/S Phases, Regulation of Cell Size