E2F3 Antikörper (C-Term)

Produktnummer ABIN214098

Dieser Anti-E2F3 Antikörper ist ein Kaninchen Polyklonal Antikörper zur Detektion von E2F3 in WB, IHC (p) und IHC. Geeignet für Human, Maus, Ratte, Rind (Kuh), Hund, Meerschweinchen, Pferd, Fledermaus, Schwein und Hamster.

Kurzübersicht für E2F3 Antikörper (C-Term) (ABIN214098)

Target: E2F3

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Hund, Meerschweinchen, Pferd, Fledermaus, Schwein, Hamster

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser E2F3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC)

Produktdetails anti-E2F3 Antikörper

Bindungsspezifität: C-Term

Spezifität: Human E2F3

Homologie: Percent identity by BLAST analysis: Human, Mouse, Dog, Bovine, Pig (100%) Rabbit, Chicken (92%) Xenopus (84%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human E2F3 (Q68DT0). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Mouse, Rat, Hamster, Elephant, Dog, Bovine, Bat, Horse, Pig, Opossum, Guinea pig (100%), Marmoset, Rabbit, Turkey, Zebra finch, Chicken, Platypus, Lizard (92%), Panda (90%), Xenopus (84%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled water

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu E2F3

Target: E2F3

Hintergrund: Name/Gene ID: E2F3

Synonyms: E2F3, E2F transcription factor 3, KIAA0075, Transcription factor E2F3, E2F-3

Gen-ID: 1871

UniProt: O00716