E2F2 Antikörper (AA 191-240)

Produktnummer ABIN6736712

Der Kaninchen Polyklonal Anti-E2F2-Antikörper wurde für WB, IHC und IHC (p) validiert. Er ist geeignet, E2F2 in Proben von Human, Maus, Kaninchen, Hund, Rind (Kuh), Pferd, Affe und Fledermaus zu detektieren.

Kurzübersicht für E2F2 Antikörper (AA 191-240) (ABIN6736712)

Target: E2F2 (E2F Transcription Factor 2 (E2F2))

Reaktivität: Human, Maus, Kaninchen, Hund, Rind (Kuh), Pferd, Affe, Fledermaus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser E2F2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-E2F2 Antikörper

Bindungsspezifität: AA 191-240

Marke: IHC-plus™

Spezifität: Human E2F2

Homologie: Percent identity by BLAST analysis: Human, Dog, Bovine, Horse (100%) Guinea pig (92%) Mouse, Rabbit (85%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa191-240 of human E2F2 (Q14209, NP_004082). Percent identity by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Panda, Dog, Bovine, Bat, Horse (100%), Gorilla, Galago, Elephant, Guinea pig (92%), Mouse, Rabbit (85%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu E2F2

Target: E2F2 (E2F Transcription Factor 2 (E2F2))

Andere Bezeichnung: E2F2

Hintergrund: Name/Gene ID: E2F2

Synonyms: E2F2, E2F-2, E2F transcription factor 2, Transcription factor E2F2

Gen-ID: 1870

NCBI Accession: NP_004082

UniProt: Q14209

Pathways: Zellzyklus, Mitotic G1-G1/S Phases, DNA Replication