Chromogranin A Antikörper (C-Term)

Produktnummer ABIN214090

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Chromogranin A in IHC, WB und IHC (p). Er zeigt eine Reaktivität gegenüber Human, Ratte, Maus, Affe, Schwein, Rind (Kuh), Pferd, Kaninchen, Hund, Meerschweinchen und Hamster.

Kurzübersicht für Chromogranin A Antikörper (C-Term) (ABIN214090)

Target: Chromogranin A (CHGA)

Reaktivität: Human, Ratte, Maus, Affe, Schwein, Rind (Kuh), Pferd, Kaninchen, Hund, Meerschweinchen, Hamster

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Chromogranin A Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-Chromogranin A Antikörper

Bindungsspezifität: C-Term

Marke: IHC-plus™

Spezifität: Human CHGA / Chromogranin A

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat, Rabbit, Horse, Pig, Guinea pig (100%) Chicken (92%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human CHGA (P10645, NP_001266). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Bovine, Rabbit, Horse, Pig, Guinea pig (100%), Opossum, Turkey, Zebra finch, Chicken, Lizard (92%), Platypus (85%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu Chromogranin A

Target: Chromogranin A (CHGA)

Andere Bezeichnung: CHGA / Chromogranin A

Hintergrund: Name/Gene ID: CHGA

Synonyms: CHGA, Chromogranin A, Chromogranin-A, Pancreastatin, Pituitary secretory protein I, Vasostatin I, SP-I, Vasostatin II

Gen-ID: 1113

NCBI Accession: NP_001266

UniProt: P10645

Pathways: Negative Regulation of Hormone Secretion, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling