LEF1 Antikörper (AA 51-100)

Produktnummer ABIN6736414

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch LEF1 in WB, IHC und IHC (p). Er zeigt eine Reaktivität gegenüber Human, Hund, Meerschweinchen, Rind (Kuh) und Affe.

Kurzübersicht für LEF1 Antikörper (AA 51-100) (ABIN6736414)

Target: LEF1 (Lymphoid Enhancer-Binding Factor 1 (LEF1))

Reaktivität: Human, Hund, Meerschweinchen, Rind (Kuh), Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser LEF1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-LEF1 Antikörper

Bindungsspezifität: AA 51-100

Marke: IHC-plus™

Spezifität: Human LEF1

Homologie: Percent identity by BLAST analysis: Human, Dog, Bovine (100%) Pig (92%) Guinea pig (85%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa51-100 of human LEF1 (Q9UJU2, NP_057353). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Elephant, Panda, Dog, Bovine (100%), Galago, Rabbit, Pig, Opossum (92%), Guinea pig (85%), Mouse, Rat (78%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (0.1 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute with 50 μL sterile ddH2O.

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu LEF1

Target: LEF1 (Lymphoid Enhancer-Binding Factor 1 (LEF1))

Andere Bezeichnung: LEF1

Hintergrund: Name/Gene ID: LEF1

Synonyms: LEF1, LEF-1, TCF7L3, TCF1-alpha, TCF10, TCF1ALPHA

Gen-ID: 51176

NCBI Accession: NP_057353

UniProt: Q9UJU2

Pathways: WNT Signalweg, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Hormone Metabolic Process, Nuclear Hormone Receptor Binding, Chromatin Binding