FOXO3 Antikörper (C-Term)

Produktnummer ABIN6736383

Der Kaninchen Polyklonal Anti-FOXO3-Antikörper wurde für WB, IHC und IHC (p) validiert. Er ist geeignet, FOXO3 in Proben von Human, Maus, Ratte, Hund, Pferd, Huhn, Meerschweinchen, Affe, Fledermaus und Hamster zu detektieren.

Kurzübersicht für FOXO3 Antikörper (C-Term) (ABIN6736383)

Target: FOXO3 (Forkhead Box O3 (FOXO3))

Reaktivität: Human, Maus, Ratte, Hund, Pferd, Huhn, Meerschweinchen, Affe, Fledermaus, Hamster

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser FOXO3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-FOXO3 Antikörper

Bindungsspezifität: C-Term

Marke: IHC-plus™

Spezifität: Human FOXO3 / FOXO3A

Homologie: Percent identity by BLAST analysis: Pig (92%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human FOXO3 (Q68DV3). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Dog, Bat, Horse, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Lizard (100%), Galago, Sheep, Bovine, Rabbit, Pig, Platypus (92%), Xenopus (84%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (2.5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu FOXO3

Target: FOXO3 (Forkhead Box O3 (FOXO3))

Andere Bezeichnung: FOXO3 / FOXO3A

Hintergrund: Name/Gene ID: FOXO3
Family: Transcription factor

Synonyms: FOXO3, AF6q21, AF6q21 protein, Forkhead box O3, Forkhead box O3A, FOXO3A, FKHRL1P2, Forkhead box protein O3, FOXO2, FKHRL1

Gen-ID: 2309

UniProt: O43524

Pathways: Zellzyklus, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Carbohydrate Homeostasis