CXCL12 Antikörper (C-Term)

Produktnummer ABIN214748

Der Kaninchen Polyklonal Anti-CXCL12-Antikörper wurde für WB, IHC und IHC (p) validiert. Er ist geeignet, CXCL12 in Proben von Human, Maus, Hund, Huhn, Xenopus laevis, Fledermaus, Meerschweinchen, Pferd und Affe zu detektieren.

Kurzübersicht für CXCL12 Antikörper (C-Term) (ABIN214748)

Target: CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))

Reaktivität: Human, Maus, Hund, Huhn, Xenopus laevis, Fledermaus, Meerschweinchen, Pferd, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CXCL12 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-CXCL12 Antikörper

Bindungsspezifität: C-Term

Marke: IHC-plus™

Spezifität: Human CXCL12

Homologie: Percent identity by BLAST analysis: Dog, Pig, Chicken (100%) Rat, Bovine (90%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human CXCL12 (P48061, NP_000600). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Dog, Cat, Bat, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Xenopus (100%), Rat, Bovine (90%), Elephant (81%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled water

Konzentration: Lot specific

Buffer: Lyophilized from PBS buffer with 2 % sucrose

Handhabung: avoid freeze thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu CXCL12

Target: CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))

Andere Bezeichnung: SDF-1 / CXCL12

Hintergrund: Name/Gene ID: CXCL12
Subfamily: Intercrine alpha
Family: Intercrine

Synonyms: CXCL12, C-X-C motif chemokine 12, HIRH, IRH, SDF-1, SDF-1b, SDF1A, SDF1B, TPAR1, TLSF-a, TLSF-b, SDF-1a, SDF1, Sdf1alpha, Stromal cell-derived factor 1, TLSF, HSDF-1, PBSF, SCYB12

Gen-ID: 6387

NCBI Accession: NP_000600

UniProt: P48061

Pathways: Regulation of Cell Size, CXCR4-mediated Signaling Events, Negative Regulation of intrinsic apoptotic Signaling