BRD4 Antikörper (AA 651-700)

Produktnummer ABIN214729

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch BRD4 in WB, IHC und IHC (p). Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte, Hund, Rind (Kuh), Meerschweinchen, Kaninchen, Pferd, Schwein und Fledermaus.

Kurzübersicht für BRD4 Antikörper (AA 651-700) (ABIN214729)

Target: BRD4 (Bromodomain Containing 4 (BRD4))

Reaktivität: Human, Maus, Ratte, Hund, Rind (Kuh), Meerschweinchen, Kaninchen, Pferd, Schwein, Fledermaus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser BRD4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-BRD4 Antikörper

Bindungsspezifität: AA 651-700

Marke: IHC-plus™

Spezifität: Human BRD4

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig (100%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa651-700 of human BRD4 (O60885-2, NP_055114). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Galago, Mouse, Rat, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Platypus, Lizard (100%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (10 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu BRD4

Target: BRD4 (Bromodomain Containing 4 (BRD4))

Andere Bezeichnung: BRD4

Hintergrund: Name/Gene ID: BRD4
Subfamily: BRD
Family: Protein Kinase

Synonyms: BRD4, Bromodomain containing 4, Chromosome-associated protein, MCAP, HUNK1, Protein HUNK1, Bromodomain-containing 4, HUNKI

Gen-ID: 23476

NCBI Accession: NP_055114

UniProt: O60885

Pathways: Chromatin Binding, SARS-CoV-2 Protein Interaktom