Rekombinanter BrdU Antikörper (Sortase,His tag,AVI tag)

Produktnummer ABIN6731027

Der Sortase,His tag,AVI tag-konjugierte Maus Monoklonal anti-BrdU Antikörper (ABIN6731027) detektiert spezifisch BrdU in IF. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für Rekombinanter BrdU Antikörper (Sortase,His tag,AVI tag) (ABIN6731027)

Target: BrdU (Bromodeoxyuridine (BrdU))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser BrdU Antikörper ist konjugiert mit Sortase,His tag,AVI tag

Applikation: Immunofluorescence (IF)

Produktdetails anti-BrdU Antikörper (Sortase,His tag,AVI tag)

Verwendungszweck: AbFlex BrdU antibody (rAb)

Marke: AbFlex®

Produktmerkmale: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis Tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports.

Aufreinigung: Protein A Chromatography

Immunogen: This antibody was raised against Bromodeoxyuridine conjugated to BSA (Bovine Serum Albumin).

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: IF: 0.5 - 2 μg/mL AbFlex recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones. For details on the parental clone, see Catalog No. 61273.

Kommentare:

This recombinant antibody has been generated using defined DNA sequences to achieve high specificity and high reproducibility. The unique advantages of this antibody are its flexible labeling and purification options as it contains a sortase recognition motif (LPXTG) to covalently add fluorophores, enzymatic substrates (HRP, AP...etc), peptides, DNA, drugs or other labels to the antibody in a directed and reproducible manner. Every antibody also contains a 6xHis Tag, which can be used with nickel-based purification systems, and a Biotinylation Tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035 % sodium azide, 30 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Lagerung: -20 °C

Informationen zur Lagerung: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Haltbarkeit: 24 months

Details zu BrdU

Target: BrdU (Bromodeoxyuridine (BrdU))

Andere Bezeichnung: BrdU

Substanzklasse: Chemical

Hintergrund: AbFlex BrdU antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. BrdU (Bromodeoxyuridine) is a thymidine analogue, capable of being incorporated into DNA in place of thymidine during DNA synthesis. By pulse labeling with BrdU, the DNA synthesis in the cells can be analyzed by detection with anti-BrdU antibodies. Antibodies against BrdU recognize BrdU in single-stranded DNA (produced by partial denaturation of double-stranded DNA), as well as free BrdU or BrdU coupled to a protein carrier.