Rekombinanter CHD3 Antikörper (AA 865-885) (Sortase,His tag,AVI tag)

Produktnummer ABIN6731021

Der Sortase,His tag,AVI tag-konjugierte Maus Monoklonal anti-CHD3 Antikörper (ABIN6731021) detektiert spezifisch CHD3 in WB und IP. Dieser Antikörper reagiert spezifisch mit Proben aus Drosophila melanogaster.

Kurzübersicht für Rekombinanter CHD3 Antikörper (AA 865-885) (Sortase,His tag,AVI tag) (ABIN6731021)

Target: CHD3 (Chromodomain Helicase DNA Binding Protein 3 (CHD3))

Antikörpertyp: Recombinant Antibody

Reaktivität: Drosophila melanogaster

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CHD3 Antikörper ist konjugiert mit Sortase,His tag,AVI tag

Applikation: Western Blotting (WB), Immunoprecipitation (IP)

Produktdetails anti-CHD3 Antikörper (Sortase,His tag,AVI tag)

Bindungsspezifität: AA 865-885

Verwendungszweck: AbFlex CHD3 antibody (rAb)

Marke: AbFlex®

Produktmerkmale: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis Tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports.

Aufreinigung: Protein A Chromatography

Immunogen: This antibody was raised against a synthetic peptide corresponding to amino acid residues 865-885 of Drosophila.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: IP: 4 μg per IP WB: 0.5 - 2 μg/mL For optimal results, we recommend the addition of 0.05 % Tween 20 to all blocking solutions to reduce background. Individual optimization may be required. AbFlex recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones. For details on the parental clone, see Catalog No. 61469.

Kommentare:

This recombinant antibody has been generated using defined DNA sequences to achieve high specificity and high reproducibility. The unique advantages of this antibody are its flexible labeling and purification options as it contains a sortase recognition motif (LPXTG) to covalently add fluorophores, enzymatic substrates (HRP, AP...etc), peptides, DNA, drugs or other labels to the antibody in a directed and reproducible manner. Every antibody also contains a 6xHis Tag, which can be used with nickel-based purification systems, and a Biotinylation Tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035 % sodium azide, 30 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Lagerung: -20 °C

Informationen zur Lagerung: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Haltbarkeit: 24 months

Details zu CHD3

Target: CHD3 (Chromodomain Helicase DNA Binding Protein 3 (CHD3))

Andere Bezeichnung: CHD3

Hintergrund: AbFlex CHD3 antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. CHD3 (chromodomain helicase DNA binding protein 3) is a component of the CHD family of ATP-dependent chromatin remodelers and forms histone deacetylase NuRD complexes which participate in the remodeling of chromatin by deacetylating histones.

Molekulargewicht: 103 kDa

NCBI Accession: NP_649111