GLIS1 Antikörper

Produktnummer ABIN6731016

Dieser Ratte Monoklonal Antikörper detektiert spezifisch GLIS1 in WB und IF. Es zeigt Reaktivität gegenüber Proben von Human und Maus.

Kurzübersicht für GLIS1 Antikörper (ABIN6731016)

Target: GLIS1 (GLIS Family Zinc Finger 1 (GLIS1))

Reaktivität: Human, Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser GLIS1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Klon: 7-14

Produktdetails anti-GLIS1 Antikörper

Verwendungszweck: GLIS1 antibody (mAb) (Clone 7-14)

Aufreinigung: Protein G Chromatography

Immunogen: This antibody was raised against a peptide within the amino-terminal half of human GLIS1 (GLI-similar 1), which shares homology with mouse GLIS1.

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: WB*: 0.5 - 2 μg/mL IF: 1-2 μg/mL *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Lagerung: -20 °C

Informationen zur Lagerung: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Haltbarkeit: 24 months

Details zu GLIS1

Target: GLIS1 (GLIS Family Zinc Finger 1 (GLIS1))

Andere Bezeichnung: GLIS1

Hintergrund: GLIS1 (Glis Family Zinc Finger 1) belongs to a GLIS subfamily of Krüppel-like zinc-finger transcription factors characterized to regulate a set of downstream targets essential for cellular functions, including pancreatic development, b-cell maturation and maintenance, and insulin production. GLIS1 is enriched in unfertilized eggs and embryos and it can be used to promote direct reprogramming of somatic cells to induced pluripotent stem cells (iPS cells). GLIS1 may regulate the expression of numerous genes, either positively or negatively, and is implicated in mitral valve development, where dysregulation may lead to mitral valve prolapse (MVP).

Molekulargewicht: 95 kDa

NCBI Accession: NP_671726