Rekombinanter Rpb1 CTD Antikörper (pTyr1)

Produktnummer ABIN6731014

Der Maus Monoklonal Anti-Rpb1 CTD-Antikörper wurde für WB, IF und ELISA validiert. Er ist geeignet, Rpb1 CTD in Proben von Human zu detektieren.

Kurzübersicht für Rekombinanter Rpb1 CTD Antikörper (pTyr1) (ABIN6731014)

Target: Rpb1 CTD (RNA Polymerase II CTD)

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Rpb1 CTD Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), ELISA

Produktdetails anti-Rpb1 CTD Antikörper

Bindungsspezifität: pTyr1

Marke: AbFlex®

Aufreinigung: Protein A Chromatography

Immunogen: This antibody was raised against synthetic peptide containing the RNA Pol II heptad repeat consensus sequence phosphorylated at tyrosine 1.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise:
Validated Applications:
IF: 0.5 - 2 µg/ml
Bead-based ELISA: 0.5 - 10 µg/ml
WB: 1 - 2 μg/mL

For optimal results, we recommend the addition of 0.1% Tween 20 to all Blotto solutions to reduce background. Individual optimization may be required.

Kommentare:

AbFlex® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6XHis tag, an avidin tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex^® RNA Pol II CTD phospho Tyr1 antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035 % sodium azide, 30 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Details zu Rpb1 CTD

Target: Rpb1 CTD (RNA Polymerase II CTD)

Andere Bezeichnung: RNA Pol II CTD

Molekulargewicht: 210 kDa