His Tag Antikörper (Cy3)

Produktnummer ABIN6720721

Hersteller Produkt- Nr.: 200-304-382

Dieses Maus Monoklonal-Antikörper erkennt spezifisch His Tag in WB, ELISA, FACS, FM, DB und FLISA. Er zeigt eine Reaktivität gegenüber und wurde in 3+ Publikationen erwähnt.

Kurzübersicht für His Tag Antikörper (Cy3) (ABIN6720721)

Target: His Tag

Reaktivität: Bitte anfragen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser His Tag Antikörper ist konjugiert mit Cy3

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Fluorescence Microscopy (FM), Dot Blot (DB), FLISA

Klon: 33D10-D2-G8

Produktdetails anti-His Tag Antikörper (Cy3)

Hersteller Produkt- Nr.: 200-304-382

Hersteller: Rockland

Verwendungszweck: 6X His Tag CY3 Conjugated Antibody

Kreuzreaktivität (Details): This monoclonal anti-6X His tag antibody detects over-expressed proteins containing the 6X His epitope tag.

Aufreinigung: 6X HIS Epitope Tag CY3 conjugated antibody was purified from concentrated tissue culture supernate by Protein A chromatography is directed against the 6X His motif and is useful in determining its presence in various assays.

Immunogen: HIS Tag antibody was produced in mice by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.

Isotyp: IgG1 kappa

Labeling Ratio: 8.3

Anwendungsinformationen

Applikationshinweise:

FLISA_Dilution: 1:10,000 - 1:50,000

ELISA_Dilution: User Optimized

Flow_Cytometry_Dilution: 1:500 - 1:2,500

IF_Microscopy_Dilution: 1:1,000 - 1:5,000

Western_Blot_Dilution: User Optimized

Kommentare:

Suggested Applications: ELISA, Microarray
Anti-6X His is optimally suited for monitoring expression of His-tagged fusion proteins. As such, anti-6X His/6X His can be used to identify fusion proteins that contain the 6X His epitope.  The antibody recognizes the His tag fused either to the amino- or carboxy- termini of targeted proteins.  This antibody has been tested by dot blot, ELISA, and western blotting against both the immunizing peptide and His-containing recombinant proteins.  CY3s are designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 50μL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen für anti-His Tag Antikörper (Cy3) (ABIN6720721)

Keskin, Chen, Tsai, Du, Santos, Syu: "Reverse-Phase Protein Microarrays for Overexpressed Escherichia coli Lysates Reveal a Novel Tyrosine Kinase." in: Analytical chemistry, Vol. 96, Issue 21, pp. 8721-8729, (2024) (PubMed).

Srivastava, Verhagen, Sasmal, Wasik, Diaz, Yu, Bensing, Khan, Khedri, Secrest, Sullam, Varki, Chen, Parrish, Varki: "Development and applications of sialoglycan-recognizing probes (SGRPs) with defined specificities: exploring the dynamic mammalian sialoglycome." in: Glycobiology, Vol. 32, Issue 12, pp. 1116-1136, (2022) (PubMed).

Khan, Sasmal, Khedri, Secrest, Verhagen, Srivastava, Varki, Chen, Yu, Beddoe, Paton, Paton, Varki: "Sialoglycan-binding patterns of bacterial AB5 toxin B subunits correlate with host range and toxicity, indicating evolution independent of A subunits." in: The Journal of biological chemistry, Vol. 298, Issue 5, pp. 101900, (2022) (PubMed).

Details zu His Tag

Target: His Tag

Hintergrund:

Synonyms: mouse anti-6X His Tag CY3 conjugated Antibody, CY3 conjugated mouse anti-6X His Tag Antibody, anti-HIS, HIS Antibody, 6X His Tag Antibody, HHHHHH epitope tag antibody, CY3, Cyanine 3

Background: 6X His Tag CY3 conjugated Antibody as well as other Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies.  Due to their small size, epitope tags do not affect the tagged protein's biochemical properties.   Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence.  This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means.  This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein.  The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures.  Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.  Rockland Immunochemicals produces anti-epitope tag antibodies against many common epitope tags including Myc, GST, GFP, 6X His, MBP, FLAG and HA. Rockland Immunochemicals also produces antibodies to other tags including FITC, Rhodamine (TRITC), DNP and biotin.