His Tag Antikörper (DyLight 800)

Produktnummer ABIN6720719

Hersteller Produkt- Nr.: 200-345-382

Der Maus Monoklonal Anti-His Tag-Antikörper wurde für WB, ELISA, FM, DB und FLISA validiert. Er ist geeignet, His Tag in Proben von zu detektieren. Es ist 1 Publikation verfügbar.

Kurzübersicht für His Tag Antikörper (DyLight 800) (ABIN6720719)

Target: His Tag

Reaktivität: Bitte anfragen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser His Tag Antikörper ist konjugiert mit DyLight 800

Applikation: Western Blotting (WB), ELISA, Fluorescence Microscopy (FM), Dot Blot (DB), FLISA

Klon: 33D10-D2-G8

Produktdetails anti-His Tag Antikörper (DyLight 800)

Hersteller Produkt- Nr.: 200-345-382

Hersteller: Rockland

Verwendungszweck: 6X His Tag Antibody Dylight™ 800 Conjugated

Kreuzreaktivität (Details): This monoclonal anti-6X His tag antibody detects over-expressed proteins containing the 6X His epitope tag.

Aufreinigung: This protein-A purified antibody is directed against the 6X His motif and is useful in determining its presence in various assays.

Immunogen: This antibody was produced in mice by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.

Isotyp: IgG1 kappa

Labeling Ratio: 2.6

Anwendungsinformationen

Applikationshinweise:

FLISA_Dilution: >1:20,000

IF_Microscopy_Dilution: >1:5,000

Western_Blot_Dilution: 1:10,000-1:25,000

Other: User Optimized

Kommentare:

Mouse Anti-6X HIS EPITOPE TAG DyLight™800 Conjugated has been tested in ELISA, dot blot, and western blot and is optimally suited for monitoring expression of His-tagged fusion proteins. As such, anti-6X His/6X His can be used to identify fusion proteins that contain the 6X His epitope.  The antibody recognizes the His tag fused either to the amino- or carboxy- termini of targeted proteins.  This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA), fluorescent polarization, FRET and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 100 μL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Referenzen für anti-His Tag Antikörper (DyLight 800) (ABIN6720719)

Minnaert, van Schie, Grootaert, Himpe, Devos, Weyts, De Keersmaecker, Braeckmans, Van Aken, Delanghe, De Smedt, Callewaert, Remaut: "Yeast-produced fructosamine-3-kinase retains mobility after ex vivo intravitreal injection in human and bovine eyes as determined by Fluorescence Correlation Spectroscopy." in: International journal of pharmaceutics, Vol. 621, pp. 121772, (2022) (PubMed).

Details zu His Tag

Target: His Tag

Hintergrund:

Synonyms: mouse anti-6X His Tag DyLight™ 800 conjugated Antibody, DyLight™ 800 conjugated mouse anti-6X His Tag Antibody, anti-HIS, HIS Antibody, 6X His Tag Antibody, HHHHHH epitope tag antibody

Background: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA), fluorescent polarization, FRET and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.