HA-Tag Antikörper (AA 114-122) (DyLight 800)

Produktnummer ABIN6720697

Hersteller Produkt- Nr.: 600-445-384

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch HA-Tag in WB, FM und FLISA. Es wurde in 4+ Publikationen zitiert.

Kurzübersicht für HA-Tag Antikörper (AA 114-122) (DyLight 800) (ABIN6720697)

Target: HA-Tag

Reaktivität: Bitte anfragen

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HA-Tag Antikörper ist konjugiert mit DyLight 800

Applikation: Western Blotting (WB), Fluorescence Microscopy (FM), FLISA

Produktdetails anti-HA-Tag Antikörper (DyLight 800)

Bindungsspezifität: AA 114-122

Hersteller Produkt- Nr.: 600-445-384

Hersteller: Rockland

Verwendungszweck: HA Epitope Tag Antibody Dylight™ 800 Conjugated

Kreuzreaktivität (Details): The antibody recognizes the HA epitope tag (Tyr-Pro-Tyr-Asp-Val-Pro-Asp-Tyr-Ala-Gly) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.

Aufreinigung: This affinity purified antibody is directed against the HA epitope tag and is useful in determining its presence in over expressed proteins in various assays.

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding aa 114-122 (Y-P-Y-D-V-P-D-Y-A-G) of hemagglutinin influenza conjugated to KLH using maleimide.

Isotyp: IgG

Labeling Ratio: 2.0

Anwendungsinformationen

Applikationshinweise:

FLISA_Dilution: >1:20,000

IF_Microscopy_Dilution: >1:5,000

Western_Blot_Dilution: 1:10,000 - 1:25,000

Other: User Optimized

Kommentare:

Suggested Applications: IF, WB
Anti-HA Epitope Tag DyLight 800 Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 100 μL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Referenzen für anti-HA-Tag Antikörper (DyLight 800) (ABIN6720697)

Wang, Alabi, Gu, Gill, Zhang, Jarad, Xia, Shen, Wang, Zhang: "Identification of amino acid residues in the MT-loop of MT1-MMP critical for its ability to cleave low-density lipoprotein receptor." in: Frontiers in cardiovascular medicine, Vol. 9, pp. 917238, (2022) (PubMed).

Willard, Douros, Gabe, Showalter, Wainscott, Suter, Capozzi, van der Velden, Stutsman, Cardona, Urva, Emmerson, Holst, D'Alessio, Coghlan, Rosenkilde, Campbell, Sloop: "Tirzepatide is an imbalanced and biased dual GIP and GLP-1 receptor agonist." in: JCI insight, Vol. 5, Issue 17, (2021) (PubMed).

Alabi, Xia, Gu, Wang, Deng, Yang, Adijiang, Douglas, Kneteman, Xue, Chen, Qin, Wang, Zhang: "Membrane type 1 matrix metalloproteinase promotes LDL receptor shedding and accelerates the development of atherosclerosis." in: Nature communications, Vol. 12, Issue 1, pp. 1889, (2021) (PubMed).

Waizenegger, Urulangodi, Lehmann, Reyes, Saugar, Tercero, Szakal, Branzei: "Mus81-Mms4 endonuclease is an Esc2-STUbL-Cullin8 mitotic substrate impacting on genome integrity." in: Nature communications, Vol. 11, Issue 1, pp. 5746, (2020) (PubMed).

Details zu HA-Tag

Target: HA-Tag

Andere Bezeichnung: HA Epitope Tag

Substanzklasse: Tag

Hintergrund:

Synonyms: Rabbit Anti-HA Epitope Tag Antibody DyLight 800™ Conjugated, Rabbit Anti HA Epitope Tag Antibody DyLight 800™ Conjugated

Background: Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the biochemical properties of the tagged protein. Most often, sequences encoding the epitope tag are included with the target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows Anti epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. HA tag is frequently incorporated into recombinant proteins for a variety of purposes. An anti-HA antibody can then be used to detect the protein when doing studies with transfected cells.