Fluorescein Antikörper (DyLight 549)

Produktnummer ABIN6720646

Hersteller Produkt- Nr.: 600-142-096

Dieser Ziege Polyklonal Antikörper detektiert spezifisch Fluorescein in WB, FLISA und FM.

Kurzübersicht für Fluorescein Antikörper (DyLight 549) (ABIN6720646)

Target: Fluorescein

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: DyLight 549

Applikation: Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)

Produktdetails

Hersteller Produkt- Nr.: 600-142-096

Hersteller: Rockland

Verwendungszweck: Fluorescein Antibody Dylight™ 549 Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and Fluorescein conjugated Bovine Serum Albumin.

Aufreinigung: Fluorescein Antibody Dylight™ 549 Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Immunogen: Fluorescein conjugated to Goat IgG

Isotyp: IgG

Labeling Ratio: 5.6

Anwendungsinformationen

Applikationshinweise:

FLISA_Dilution: >1:20,000

IF_Microscopy_Dilution: >1:5,000

Western_Blot_Dilution: 1:10,000 - 1:25,000

Other: User Optimized

Kommentare:

Anti-Fluorescein Antibody Dylight™ 549 Conjugated is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 100 μL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Antigendetails

Target: Fluorescein

Substanzklasse: Chemical

Hintergrund:

Synonyms: goat Anti-FITC antibody DyLight™ 549 conjugation, DyLight™ 549 conjugated goat anti-fluorescein antibody, DyLight™549 conjugated goat Anti-FITC antibody, goat anti-fluorescein antibody DyLight™ 549 conjugation

Background: Fluorescence technology is widely used to detect proteins. However, many common visible fluorophores often result in considerable background fluorescence in the visible range. Visible fluorophores are rarely used for membrane-based protein detection because of this high background. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation. Dylight™ antibody and reagent conjugates are specifically designed for protein detection methods that covers much of the visible spectrum and into the near-infrared region to visualize proteins in western blotting and other applications. Very low background fluorescence in the IR range provides for a much higher signal-to-noise ratio than visible fluorophores. Detection levels in the picogram range on Western blots rival the sensitivity of chemiluminescence on film. Dylight™conjugates provide an ultra-sensitive and convenient alternative to standard chemiluminescent protein detection methods, as well as a valuable tool for multicolor imaging.