His Tag Antikörper (DyLight 649)

Produktnummer ABIN6720637

Hersteller Produkt- Nr.: 200-343-382

Dieses Maus Monoklonal-Antikörper erkennt spezifisch His Tag in WB, ELISA, FM, DB und FLISA. Er zeigt eine Reaktivität gegenüber und wurde in 5+ Publikationen erwähnt.

Kurzübersicht für His Tag Antikörper (DyLight 649) (ABIN6720637)

Target: His Tag

Reaktivität: Bitte anfragen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser His Tag Antikörper ist konjugiert mit DyLight 649

Applikation: Western Blotting (WB), ELISA, Fluorescence Microscopy (FM), Dot Blot (DB), FLISA

Klon: 33D10-D2-G8

Produktdetails anti-His Tag Antikörper (DyLight 649)

Hersteller Produkt- Nr.: 200-343-382

Hersteller: Rockland

Verwendungszweck: 6X His Tag Antibody DyLight™ 649

Kreuzreaktivität (Details): This monoclonal anti-6X His tag antibody detects over-expressed proteins containing the 6X His epitope tag.

Aufreinigung: This protein-A purified antibody is directed against the 6X His motif and is useful in determining its presence in various assays.

Immunogen: This antibody was produced in mice by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.

Isotyp: IgG1 kappa

Labeling Ratio: 2.5

Anwendungsinformationen

Applikationshinweise:

FLISA_Dilution: >1:20,000

IF_Microscopy_Dilution: >1:5,000

Western_Blot_Dilution: >1:10,000-1:25,000

Other: FLUORESCENT POLARIZATION: 2 - 20 nM

Kommentare:

Suggested Applications: Biochemical Assay, Microarray
Anti-6X HIS tagged Antibody conjugated to DyLight™649 has been tested by ELISA, dot blot, and western blot. This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA), fluorescent polarization, FRET and fluorescent western blotting. Anti-6X HIS Antibody is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 100 μL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Referenzen für anti-His Tag Antikörper (DyLight 649) (ABIN6720637)

Daguer, Gonse, Shchukin, Farrera-Soler, Barluenga, Winssinger: "Dual Bcl-XL /Bcl-2 inhibitors discovered from DNA-encoded libraries using a fragment pairing strategy." in: Bioorganic & medicinal chemistry, Vol. 44, pp. 116282, (2021) (PubMed).

Hunter, McIntosh, Shi, Sperberg, Funatogawa, Labanieh, Soon, Wastyk, Mehta, Carter, Hunter, Cochran: "An engineered ligand trap inhibits leukemia inhibitory factor as pancreatic cancer treatment strategy." in: Communications biology, Vol. 4, Issue 1, pp. 452, (2021) (PubMed).

Mehta, Maddineni, Kelly, Lee, Hunter, Silberstein, Parra Sperberg, Miller, Rabe, Labanieh, Cochran: "An engineered antibody binds a distinct epitope and is a potent inhibitor of murine and human VISTA." in: Scientific reports, Vol. 10, Issue 1, pp. 15171, (2020) (PubMed).

Kariolis, Miao, Diep, Nash, Olcina, Jiang, Jones, Kapur, Mathews, Koong, Rankin, Cochran, Giaccia: "Inhibition of the GAS6/AXL pathway augments the efficacy of chemotherapies." in: The Journal of clinical investigation, Vol. 127, Issue 1, pp. 183-198, (2017) (PubMed).

Kariolis, Miao, Jones, Kapur, Mathews, Giaccia, Cochran: "An engineered Axl 'decoy receptor' effectively silences the Gas6-Axl signaling axis." in: Nature chemical biology, Vol. 10, Issue 11, pp. 977-83, (2014) (PubMed).

Details zu His Tag

Target: His Tag

Hintergrund:

Synonyms: mouse anti-6X His Tag DyLight™ 649 conjugated Antibody, DyLight™ 649 conjugated mouse anti-6X His Tag Antibody, anti-HIS, HIS Antibody, 6X His Tag Antibody, HHHHHH epitope tag antibody

Background: Anti 6X HIS tagged Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA), fluorescent polarization, FRET and fluorescent western blotting. Anti-6X HIS Antibody is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.