Calpain 2 Antikörper (AA 500-700)

Produktnummer ABIN6719643

Der Maus Monoklonal anti-Calpain 2 Antikörper (Klon 8I6) (ABIN6719643) detektiert spezifisch Calpain 2 in WB, IHC, IF, ICC und FACS. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus und Affe.

Kurzübersicht für Calpain 2 Antikörper (AA 500-700) (ABIN6719643)

Target: Calpain 2 (CAPN2)

Reaktivität: Human, Maus, Affe

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Calpain 2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Klon: 8I6

Produktdetails anti-Calpain 2 Antikörper

Bindungsspezifität: AA 500-700

Verwendungszweck: Anti-Calpain 2 CAPN2 Antibody (monoclonal, 8I6)

Spezifität: No cross reactivity with other proteins.

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Homologie: coli-derived human Calpain 2 recombinant protein (Position: R500-L700). Human Calpain 2 shares 93.5%,92.5% amino acid (aa) sequence identity with mouse,rat Calpain 2,respectively.

Produktmerkmale: Anti-Calpain 2 CAPN2 Antibody.(monoclonal, 8I6). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse.

Aufreinigung: Immunogen affinity purified.

Immunogen: E. coli-derived human Calpain 2 recombinant protein (Position: R500-L700). Human Calpain 2 shares 93.5% and 92.5% amino acid (aa) sequence identity with mouse and rat Calpain 2, respectively.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL Immunocytochemistry/Immunofluorescence, 2 μg/mL Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells

Kommentare:

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu Calpain 2

Target: Calpain 2 (CAPN2)

Andere Bezeichnung: CAPN2

Hintergrund:

Background: Calpain-2 catalytic subunit is a protein that in humans is encoded by the CAPN2 gene. The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 2. Multiple heterogeneous transcriptional start sites in the 5' UTR have been reported.

Gene Full Name: calpain 2

Molekulargewicht: 80 kDa

Gen-ID: 824

UniProt: P17655