Melanoma gp100 Antikörper (N-Term)

Produktnummer ABIN6719641

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch Melanoma gp100 in IHC, WB, FACS und IF. Es zeigt Reaktivität gegenüber Proben von Human, Ratte und Maus.

Kurzübersicht für Melanoma gp100 Antikörper (N-Term) (ABIN6719641)

Target: Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Melanoma gp100 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF)

Produktdetails anti-Melanoma gp100 Antikörper

Bindungsspezifität: N-Term

Verwendungszweck: Anti-Melanoma gp100/PMEL Antibody

Sequenz: KVPRNQDWLG VSRQLRTKAW NRQLYPEWTE AQRLD

Spezifität: No cross reactivity with other proteins.

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-Melanoma gp100/PMEL Antibody. Tested in Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Melanoma gp100/PMEL, which shares 71.4% and 65.7% amino acid (aa) sequence identity with mouse and rat Melanoma gp100/PMEL, respectively.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human Immunofluorescence, 5 μg/mL, Human Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells, Human

Kommentare:

Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu Melanoma gp100

Target: Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

Andere Bezeichnung: PMEL

Hintergrund:

Background: This gene is mapped to 12q13.2. It encodes a melanocyte-specific type I transmembrane glycoprotein. The encoded protein is enriched in melanosomes, which are the melanin-producing organelles in melanocytes, and plays an essential role in the structural organization of premelanosomes. This protein is involved in generating internal matrix fibers that define the transition from Stage I to Stage II melanosomes. This protein undergoes a complex pattern of prosttranslational processing and modification that is essential to the proper functioning of the protein. A secreted form of this protein that is released by proteolytic ectodomain shedding may be used as a melanoma-specific serum marker. Alternate splicing results in multiple transcript variants.

Gene Full Name: premelanosome protein

Molekulargewicht: 80 kDa

UniProt: P40967