USP7 Antikörper (AA 258-483)

Produktnummer ABIN6719603

Der Maus Monoklonal Anti-USP7-Antikörper wurde für WB, IHC, IF, ICC und FACS validiert. Er ist geeignet, USP7 in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für USP7 Antikörper (AA 258-483) (ABIN6719603)

Target: USP7 (Ubiquitin Specific Peptidase 7 (Herpes Virus-Associated) (USP7))

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser USP7 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Klon: 5E2

Produktdetails anti-USP7 Antikörper

Bindungsspezifität: AA 258-483

Verwendungszweck: Anti-HAUSP/USP7 Antibody Picoband® (monoclonal, 5E2)

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-HAUSP/USP7 Antibody Picoband® (monoclonal, 5E2) (ABIN6719603). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human HAUSP/USP7 recombinant protein (Position: L258-D483).

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunocytochemistry/Immunofluorescence, 2 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells
1."Entrez Gene: USP7 ubiquitin specific peptidase 7 (herpes virus-associated)". 2.Holowaty MN, Sheng Y, Nguyen T, Arrowsmith C, Frappier L (Nov 2003). "Protein interaction domains of the ubiquitin-specific protease, USP7/HAUSP". The Journal of Biological Chemistry. 278 (48): 47753-61. 3.Li M, Chen D, Shiloh A, Luo J, Nikolaev AY, Qin J, Gu W (Apr 2002). "Deubiquitination of p53 by HAUSP is an important pathway for p53 stabilization". Nature. 416 (6881): 648-53.

Kommentare:

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu USP7

Target: USP7 (Ubiquitin Specific Peptidase 7 (Herpes Virus-Associated) (USP7))

Andere Bezeichnung: USP7

Hintergrund:

Synonyms: Ubiquitin carboxyl-terminal hydrolase 7, Deubiquitinating enzyme 7, Herpesvirus-associated ubiquitin-specific protease, Ubiquitin thioesterase 7, Ubiquitin-specific-processing protease 7

Tissue Specificity: Widely expressed. Overexpressed in prostate cancer.

Background: Ubiquitin-specific-processing protease 7 (USP7), also known as ubiquitin carboxyl-terminal hydrolase 7 or herpesvirus-associated ubiquitin-specific protease (HAUSP), is an enzyme that in humans is encoded by the USP7 gene. The protein encoded by this gene belongs to the peptidase C19 family, which includes ubiquitinyl hydrolases. This protein deubiquitinates target proteins such as p53 (a tumor suppressor protein) and WASH (essential for endosomal protein recycling), and regulates their activities by counteracting the opposing ubiquitin ligase activity of proteins such as HDM2 and TRIM27, involved in the respective process. Mutations in this gene have been implicated in a neurodevelopmental disorder.

Molekulargewicht: 140 kDa

Gen-ID: 7874

UniProt: Q93009