BCL9L Antikörper (AA 397-666)

Produktnummer ABIN6719451

Dieser Anti-BCL9L-Antikörper ist ein Kaninchen-Polyklonal-Antikörper zum Nachweis von BCL9L in WB, ELISA, ICC, IF, FACS und IHC. Geeignet für Human, Maus und Ratte.

Kurzübersicht für BCL9L Antikörper (AA 397-666) (ABIN6719451)

Target: BCL9L (B-Cell CLL/lymphoma 9-Like (BCL9L))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser BCL9L Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (IHC)

Produktdetails anti-BCL9L Antikörper

Bindungsspezifität: AA 397-666

Verwendungszweck: Anti-BCL9L Antibody

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-BCL9L Antibody (ABIN6719451). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human BCL9L recombinant protein (Position: E397-A666).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -

Kommentare:

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu BCL9L

Target: BCL9L (B-Cell CLL/lymphoma 9-Like (BCL9L))

Andere Bezeichnung: BCL9L

Hintergrund:

Synonyms: B-cell CLL/lymphoma 9-like protein, B-cell lymphoma 9-like protein, BCL9-like protein, Protein BCL9-2, BCL9L, DLNB11

Tissue Specificity: Expressed in breast, ductal and invasive ductal carcinomas of the breast, sporadic colorectal adenomas and carcinomas (at protein level). Expressed in fetal brain. Expressed in lung, amygdala, eye, prostate, pancreatic and prostate cancers, head and neck tumors and embryonal tumor.

Background: B-cell CLL/lymphoma 9 like is a protein that in humans is encoded by the BCL9L gene. It is mapped to 11q23.3. Bcl-9 (B-cell lymphoma 9, also Protein legless homolog) is a transcriptional regulator that belongs to the Bcl-9 family of proteins. It is expressed in multiple tissues and serves to recruit Pygopus to the Wnt-pathway beta -catenin-TCF complex in the nucleus. Bcl-9 and Bcl-9-2 are considered evolutionary duplicates of Legless that perform the same task with different regulation. Human Bcl-9 is 1426 amino acids (aa) in length. It contains one phosphothreonine and three phosphoserine sites that have been identified so far, two poly-Pro regions (aa 514-517 and 970-973), and one poly-Ala segment (aa 900-903). There is one potential alternate start site at Met27, and a variant isoform exists that shows a four aa substitution for aa 13911426. Over aa 1009-1328, human Bcl-9 is 96 % aa identical to mouse Bcl-9.

Molekulargewicht: 200 kDa

Gen-ID: 283149

Pathways: Stem Cell Maintenance