ACLY Antikörper (AA 1-180)

Produktnummer ABIN6719406

Dieser Anti-ACLY Antikörper ist ein Maus Monoklonal Antikörper zur Detektion von ACLY in WB, IHC, IF, FACS und ICC. Geeignet für Human, Maus und Ratte.

Kurzübersicht für ACLY Antikörper (AA 1-180) (ABIN6719406)

Target: ACLY (ATP Citrate Lyase (ACLY))

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ACLY Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Klon: 5I2

Produktdetails anti-ACLY Antikörper

Bindungsspezifität: AA 1-180

Verwendungszweck: Anti-ATP citrate lyase ACLY Antibody (monoclonal, 5I2)

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-ATP citrate lyase ACLY Antibody (monoclonal, 5I2) (ABIN6719406). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Aufreinigung: Immunogen affinity purified.

Immunogen: E. coli-derived human ATP citrate lyase recombinant protein (Position: M1-I180). Human ATP citrate lyase shares 95% amino acid (aa) sequence identity with both mouse and rat ATP citrate lyase.

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells

Kommentare:

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu ACLY

Target: ACLY (ATP Citrate Lyase (ACLY))

Andere Bezeichnung: ACLY

Hintergrund:

Synonyms: ATP-citrate synthase, ATP-citrate (pro-S-)-lyase, ACL, Citrate cleavage enzyme, ACLY

Tissue Specificity: Widely expressed. Highest level in placenta, liver, muscle and kidney.

Background: ATP citrate lyase, aslo known as ACLY, is an enzyme that in animals represents an important step in fatty acid biosynthesis. ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer of apparently identical subunits. The product, acetyl-CoA, in animals serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. It is activated by insulin. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine.In plants, ATP citrate lyase generates the acetyl-CoA for cytosolically-synthesized metabolites.

Molekulargewicht: 121 kDa

Gen-ID: 47

UniProt: P53396

Pathways: Warburg Effekt