Cytochrome C Antikörper (AA 2-105) (DyLight 550)

Produktnummer ABIN6719402

Der Maus Monoklonal Anti-Cytochrome C-Antikörper wurde für FACS validiert. Er ist geeignet, Cytochrome C in Proben von Human zu detektieren.

Kurzübersicht für Cytochrome C Antikörper (AA 2-105) (DyLight 550) (ABIN6719402)

Target: Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Cytochrome C Antikörper ist konjugiert mit DyLight 550

Applikation: Flow Cytometry (FACS)

Klon: 15F10

Produktdetails anti-Cytochrome C Antikörper (DyLight 550)

Bindungsspezifität: AA 2-105

Verwendungszweck: Anti-Human Cytochrome C DyLight® 550 conjugated CYCS Antibody(monoclonal, 15F10)

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-Human Cytochrome C DyLight® 550 conjugated CYCS Antibody (monoclonal, 15F10) -Dyl550. Tested in Flow Cytometry applications. This antibody reacts with Human.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human Cytochrome C recombinant protein (Position: G2-E105). Human Cytochrome C shares 91% amino acid (aa) sequence identity with both mouse and rat Cytochrome C.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells1. García-Heredia JM, et al. Specific nitration of tyrosines 46 and 48 makes cytochrome c assemble a non-functional apoptosome. FEBS Lett, 2012 Jan 20. 2. Rajagopal BS, et al. The hydrogen-peroxide-induced radical behaviour in human cytochrome c-phospholipid complexes: implications for the enhanced pro-apoptotic activity of the G41S mutant. Biochem J, 2013 Dec 15. 3. Simon M, et al. Versatility of non-native forms of human cytochrome c: pH and micellar concentration dependence. J Biol Inorg Chem, 2013 Jan.

Kommentare:

Other applications have not been tested. Optimal dilutions should be determined by end users.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Details zu Cytochrome C

Target: Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))

Andere Bezeichnung: CYCS

Hintergrund:

Synonyms: Cytochrome c, CYCS, CYC

Background: CYCS is also known as CYC, HCS or THC4. This gene encodes a small heme protein that functions as a central component of the electron transport chain in mitochondria. The encoded protein associates with the inner membrane of the mitochondrion where it accepts electrons from cytochrome b and transfers them to the cytochrome oxidase complex. This protein is also involved in initiation of apoptosis. Mutations in this gene are associated with autosomal dominant nonsyndromic thrombocytopenia. Numerous processed pseudogenes of this gene are found throughout the human genome.

Molekulargewicht: 39 kDa

Gen-ID: 54205

UniProt: P99999

Pathways: Apoptose, Caspase Kaskade in der Apoptose, Positive Regulation of Endopeptidase Activity