KIF15 Antikörper (AA 1201-1388)

Produktnummer ABIN6719385

Der Kaninchen Polyklonal anti-KIF15 Antikörper (ABIN6719385) detektiert spezifisch KIF15 in ELISA, WB, IF, IHC, FACS und ICC. Dieser Antikörper reagiert spezifisch mit Proben aus Human und Ratte.

Kurzübersicht für KIF15 Antikörper (AA 1201-1388) (ABIN6719385)

Target: KIF15 (Kinesin Family Member 15 (KIF15))

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser KIF15 Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Produktdetails anti-KIF15 Antikörper

Bindungsspezifität: AA 1201-1388

Verwendungszweck: Anti-KIF15 Antibody

Spezifität: No cross reactivity with other proteins.

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-KIF15 Antibody. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Rat.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human KIF15 recombinant protein (Position: M1201-S1388).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL Immunocytochemistry/Immunofluorescence, 5 μg/mL Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells ELISA, 0.1-0.5 μg/mL

Kommentare:

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu KIF15

Target: KIF15 (Kinesin Family Member 15 (KIF15))

Andere Bezeichnung: KIF15

Hintergrund:

Background: Kinesin family member 15 is a protein that in humans is encoded by the KIF15 gene. It is mapped to 3p21.31. This gene encodes a motor protein that is part of the kinesin superfamily. KIF15 maintains half spindle separation by opposing forces generated by other motor proteins. KIF15 co-localizes with microtubules and actin filaments in both dividing cells and in postmitotic neurons. KIF15 (also known as Kinesin-12 and HKLP2) is a motor protein expressed in all cells during mitosis and in postmitotic neurons undergoing axon growth. KIF15 maintains bipolar microtubule spindle apparatus in dividing cells and shares redundant functions with KIF11. KIF15 is thought to promote spindle assembly by cross-linking and sliding along microtubules creating a separation between centrosomes. HeLa cells depleted of KIF11, with reduced microtubule dynamics, are able to form bipolar spindles from acentrosomal asters in a KIF15 dependent manner. Hence, inhibition of KIF15 function will be a vital therapeutic approach in cancer chemotherapy.

Gene Full Name: kinesin family member 15

Molekulargewicht: 150-160 kDa

Gen-ID: 56992

UniProt: Q9NS87