Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

KIF15 Antikörper (AA 1201-1388)

Dieses Anti-KIF15-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von KIF15 in ELISA, WB, IF, IHC, FACS und ICC. Geeignet für Human und Ratte.
Produktnummer ABIN6719385

Kurzübersicht für KIF15 Antikörper (AA 1201-1388) (ABIN6719385)

Target

Alle KIF15 Antikörper anzeigen
KIF15 (Kinesin Family Member 15 (KIF15))

Reaktivität

  • 23
  • 9
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
Human, Ratte

Wirt

  • 20
  • 3
Kaninchen

Klonalität

  • 21
  • 2
Polyklonal

Konjugat

  • 14
  • 3
  • 2
  • 2
  • 1
  • 1
Dieser KIF15 Antikörper ist unkonjugiert

Applikation

ELISA, Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC)
  • Bindungsspezifität

    • 7
    • 6
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 1201-1388

    Verwendungszweck

    Anti-KIF15 Antibody Picoband®

    Kreuzreaktivität (Details)

    No cross-reactivity with other proteins.

    Produktmerkmale

    Anti-KIF15 Antibody Picoband® (ABIN6719385). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

    Aufreinigung

    Immunogen affinity purified.

    Immunogen

    E.coli-derived human KIF15 recombinant protein (Position: M1201-S1388).

    Isotyp

    IgG
  • Applikationshinweise

    Western blot, 0.1-0.5 μg/mL
    Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
    Immunocytochemistry/Immunofluorescence, 5 μg/mL
    Flow Cytometry (Fixed), 1-3 μg/1x106 cells
    ELISA, 0.1-0.5 μg/mL
    1. Eskova, A., Knapp, B., Matelska, D., Reusing, S., Arjonen, A., Lisauskas, T., Pepperkok, R., Russell, R., Eils, R., Ivaska, J., Kaderali, L., Erfle, H., Starkuviene, V. An RNAi screen identifies KIF15 as a novel regulator of the endocytic trafficking of integrin. J. Cell Sci. 127: 2433-2447, 2014. 2. Tanenbaum, M. E., Macurek, L., Janssen, A., Geers, E. F., Alvarez-Fernandez, M., Medema, R. H. Kif15 cooperates with Eg5 to promote bipolar spindle assembly. Curr. Biol. 19: 1703-1711, 2009.

    Kommentare

    Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    Konzentration

    500 μg/mL

    Buffer

    Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
  • Target

    KIF15 (Kinesin Family Member 15 (KIF15))

    Andere Bezeichnung

    KIF15

    Hintergrund

    Synonyms: Kinesin-like protein KIF15, Kinesin-like protein 2, hKLP2, Kinesin-like protein 7, Serologically defined breast cancer antigen NY-BR-62, KIF15, KLP2, KNSL7

    Tissue Specificity: Expressed in testis, colon, thymus and in breast cancer.

    Background: Kinesin family member 15 is a protein that in humans is encoded by the KIF15 gene. It is mapped to 3p21.31. This gene encodes a motor protein that is part of the kinesin superfamily. KIF15 maintains half spindle separation by opposing forces generated by other motor proteins. KIF15 co-localizes with microtubules and actin filaments in both dividing cells and in postmitotic neurons. KIF15 (also known as Kinesin-12 and HKLP2) is a motor protein expressed in all cells during mitosis and in postmitotic neurons undergoing axon growth. KIF15 maintains bipolar microtubule spindle apparatus in dividing cells and shares redundant functions with KIF11. KIF15 is thought to promote spindle assembly by cross-linking and sliding along microtubules creating a separation between centrosomes. HeLa cells depleted of KIF11, with reduced microtubule dynamics, are able to form bipolar spindles from acentrosomal asters in a KIF15 dependent manner. Hence, inhibition of KIF15 function will be a vital therapeutic approach in cancer chemotherapy.

    Molekulargewicht

    150-160 kDa

    Gen-ID

    56992
Sie sind hier:
Chat with us!