DKK1 Antikörper (AA 32-266)

Produktnummer ABIN6719383

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch DKK1 in WB. Er zeigt eine Reaktivität gegenüber Human, Ratte und Maus.

Kurzübersicht für DKK1 Antikörper (AA 32-266) (ABIN6719383)

Target: DKK1 (Dickkopf Homolog 1 (DKK1))

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser DKK1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-DKK1 Antikörper

Bindungsspezifität: AA 32-266

Verwendungszweck: Anti-DKK1 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-DKK1 Antibody Picoband® (ABIN6719383). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E. coli-derived human DKK1 recombinant protein (Position: T32-H266).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL
1. Fedi, P., Bafico, A., Soria, A. N., Burgess, W. H., Miki, T., Bottaro, D. P., Kraus, M. H., Aaronson, S. A.Isolation and biochemical characterization of the human Dkk-1 homologue, a novel inhibitor of mammalian Wnt signaling.J. Biol. Chem. 274: 19465-19472, 1999. 2. Roessler, E., Du, Y., Glinka, A., Dutra, A., Niehrs, C., Muenke, M.The genomic structure, chromosome location, and analysis of the human DKK1 head inducer gene as a candidate for holoprosencephaly. Cytogenet. Cell Genet. 89: 220-224, 2000. 3. Tian, E., Zhan, F., Walker, R., Rasmussen, E., Ma, Y., Barlogie, B., Shaughnessy, J. D., Jr.The role of Wnt-signaling antagonist DKK1 in the development of osteolytic lesions in multiple myeloma.New Eng. J. Med. 349: 2483-2494, 2003.

Kommentare:

Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu DKK1

Target: DKK1 (Dickkopf Homolog 1 (DKK1))

Andere Bezeichnung: DKK1

Hintergrund:

Synonyms: Dickkopf-related protein 1, Dickkopf-1, Dkk-1, hDkk-1, SK, DKK1, UNQ492/PRO1008

Tissue Specificity: Placenta.

Background: DKK1, Dickkopf-related protein 1, is a protein that in humans is encoded by the DKK1 gene. This gene encodes a protein that is a member of the dickkopf family. By genomic sequence analysis, determined that the DKK1 gene contains 4 exons. The DKK1 gene is mapped to 10q11.2 using FISH. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated levels of DKK1 in bone marrow plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma.

Molekulargewicht: 38 kDa

Gen-ID: 22943

UniProt: O94907

Pathways: WNT Signalweg, Regulation of Muscle Cell Differentiation, Positive Regulation of fat Cell Differentiation